Inheritance characteristics of microsatellite DNA loci in experimental families of Japanese flounder Paralichthys olivaceus

Masashi Sekino, Motoyuki Hara

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Allele segregating patterns of microsatellite DNA loci in 5 experimental families of Japanese flounder Paralichthys olivaceus and genotype frequencies for deviation from Hardy-Weinberg expectations (HWE) in natural P. olivaceus populations were studied to assess inheritability. Of the 12 microsatellite loci examined, 1 locus had a possibility of scoring errors of heterozygous individuals caused by unreproducible polymerase chain reaction amplifications of a particular allele. At the remaining 11 loci, almost all of alleles were segregated according to Mendelian transmission, and observed genotype frequencies in natural populations were consistent with HWE. The results demonstrated here would provide useful information supporting the suitability of these microsatellite loci as inheritable P. olivaceus genetic markers.

Original languageEnglish
Pages (from-to)310-315
Number of pages6
JournalMarine Biotechnology
Volume3
Issue number4
DOIs
Publication statusPublished - 2001 Oct 16
Externally publishedYes

Keywords

  • Japanese flounder
  • Mendelian inheritance
  • Microsatellite DNA loci
  • Paralichthys olivaceus

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

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