Inheritance characteristics of microsatellite DNA loci in experimental families of Japanese flounder Paralichthys olivaceus

Masashi Sekino; Motoyuki Hara

doi:10.1007/s10126-001-0052-z

Inheritance characteristics of microsatellite DNA loci in experimental families of Japanese flounder Paralichthys olivaceus

Masashi Sekino, Motoyuki Hara

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Allele segregating patterns of microsatellite DNA loci in 5 experimental families of Japanese flounder Paralichthys olivaceus and genotype frequencies for deviation from Hardy-Weinberg expectations (HWE) in natural P. olivaceus populations were studied to assess inheritability. Of the 12 microsatellite loci examined, 1 locus had a possibility of scoring errors of heterozygous individuals caused by unreproducible polymerase chain reaction amplifications of a particular allele. At the remaining 11 loci, almost all of alleles were segregated according to Mendelian transmission, and observed genotype frequencies in natural populations were consistent with HWE. The results demonstrated here would provide useful information supporting the suitability of these microsatellite loci as inheritable P. olivaceus genetic markers.

Original language	English
Pages (from-to)	310-315
Number of pages	6
Journal	Marine Biotechnology
Volume	3
Issue number	4
DOIs	https://doi.org/10.1007/s10126-001-0052-z
Publication status	Published - 2001 Oct 16
Externally published	Yes

Keywords

Japanese flounder
Mendelian inheritance
Microsatellite DNA loci
Paralichthys olivaceus

ASJC Scopus subject areas

Biotechnology
Aquatic Science

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10.1007/s10126-001-0052-z

Cite this

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title = "Inheritance characteristics of microsatellite DNA loci in experimental families of Japanese flounder Paralichthys olivaceus",

abstract = "Allele segregating patterns of microsatellite DNA loci in 5 experimental families of Japanese flounder Paralichthys olivaceus and genotype frequencies for deviation from Hardy-Weinberg expectations (HWE) in natural P. olivaceus populations were studied to assess inheritability. Of the 12 microsatellite loci examined, 1 locus had a possibility of scoring errors of heterozygous individuals caused by unreproducible polymerase chain reaction amplifications of a particular allele. At the remaining 11 loci, almost all of alleles were segregated according to Mendelian transmission, and observed genotype frequencies in natural populations were consistent with HWE. The results demonstrated here would provide useful information supporting the suitability of these microsatellite loci as inheritable P. olivaceus genetic markers.",

keywords = "Japanese flounder, Mendelian inheritance, Microsatellite DNA loci, Paralichthys olivaceus",

author = "Masashi Sekino and Motoyuki Hara",

year = "2001",

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doi = "10.1007/s10126-001-0052-z",

language = "English",

volume = "3",

pages = "310--315",

journal = "Marine Biotechnology",

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T1 - Inheritance characteristics of microsatellite DNA loci in experimental families of Japanese flounder Paralichthys olivaceus

AU - Sekino, Masashi

AU - Hara, Motoyuki

PY - 2001/10/16

Y1 - 2001/10/16

N2 - Allele segregating patterns of microsatellite DNA loci in 5 experimental families of Japanese flounder Paralichthys olivaceus and genotype frequencies for deviation from Hardy-Weinberg expectations (HWE) in natural P. olivaceus populations were studied to assess inheritability. Of the 12 microsatellite loci examined, 1 locus had a possibility of scoring errors of heterozygous individuals caused by unreproducible polymerase chain reaction amplifications of a particular allele. At the remaining 11 loci, almost all of alleles were segregated according to Mendelian transmission, and observed genotype frequencies in natural populations were consistent with HWE. The results demonstrated here would provide useful information supporting the suitability of these microsatellite loci as inheritable P. olivaceus genetic markers.

AB - Allele segregating patterns of microsatellite DNA loci in 5 experimental families of Japanese flounder Paralichthys olivaceus and genotype frequencies for deviation from Hardy-Weinberg expectations (HWE) in natural P. olivaceus populations were studied to assess inheritability. Of the 12 microsatellite loci examined, 1 locus had a possibility of scoring errors of heterozygous individuals caused by unreproducible polymerase chain reaction amplifications of a particular allele. At the remaining 11 loci, almost all of alleles were segregated according to Mendelian transmission, and observed genotype frequencies in natural populations were consistent with HWE. The results demonstrated here would provide useful information supporting the suitability of these microsatellite loci as inheritable P. olivaceus genetic markers.

KW - Japanese flounder

KW - Mendelian inheritance

KW - Microsatellite DNA loci

KW - Paralichthys olivaceus

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SN - 1436-2228

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ER -

Inheritance characteristics of microsatellite DNA loci in experimental families of Japanese flounder Paralichthys olivaceus

Abstract

Keywords

ASJC Scopus subject areas

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