Inhalation anesthetics induce apoptosis in normal peripheral lymphocytes in vitro

H. Matsuoka, S. Kurosawa, T. Horinouchi, M. Kato, Y. Hashimoto

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    Abstract

    Background: The authors hypothesized that perioperative lymphocytopenia is partially caused by apoptosis of lymphocytes induced by inhalation anesthetics. Therefore, they evaluated whether sevoflurane and isoflurane induce apoptosis of normal peripheral lymphocytes. Methods: Normal peripheral blood mononuclear cells were exposed to sevoflurane and isoflurane, and the percentages of apoptotic lymphocytes was measured by Annexin V-fluorescein isothiocyanate-7-amino actinomycin D flow cytometry after 24 h of exposure (0.5, 1.0, and 1.5 mM) and after 6, 12, and 24 h of exposure (1.5 mM). The percentages of lymphocytes with caspase 3-like activity were also measured after 24 h of exposure (1.5 mM). Results: The percentages of apoptotic lymhocytes were increased in a dose-dependent manner (controls: 5.1 ± 1.4%; sevoflurane: 7.3 ± 1.3% [0.5 mM], 9.1 ± 1.5% [1.0 mM], 12.6 ± 2.1% [1.5 mM]; isoflurane: 7.5 ± 1.6% [0.5 mM], 10.5 ± 1.5% [1.0 mM], 16.3 ± 2.7% [1.5 mM] after 24 h of exposure and in a time-dependent manner (controls: 1.2 ± 0.4% [6 h], 3.4 ± 0.7% [12 h], 5.6 ± 1.2% [24 h]; sevoflurane: 1.8 ± 0.4% [6 h], 6.4 ± 1.2% [12 h], 11.3 ± 2.2% [24 h]; isoflurane: 2.6 ± 0.5% [6 h], 8.8 ± 1.5% [12 h], 16.0 ± 1.9% [24 h]) at the concentration of 1.5 mM. The percentages of lymphocytes with caspase 3-like activity were increased (controls: 10.0 ± 1.1%; sevoflurane: 13.8 ± 1.2%; isoflurane: 17.0 ± 1.3%). Conclusions: Both sevoflurane and isoflurane induced apoptosis in peripheral lymphocytes in dose-dependent and time-dependent manners in vitro.

    Original languageEnglish
    Pages (from-to)1467-1472
    Number of pages6
    JournalAnesthesiology
    Volume95
    Issue number6
    DOIs
    Publication statusPublished - 2001 Jan 1

    ASJC Scopus subject areas

    • Anesthesiology and Pain Medicine

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