Infrared spectroscopy on poly(dG)-poly(dC) DNA at low hydration

Hasanudin Abdurakhman, Kenji Tajiri, Hiroyuki Yokoi, Noritaka Kuroda, Hiroshi Matsui, Takeshi Yanagimachi, Masateru Taniguchi, Tomoji Kawai, Naoki Toyota

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Infrared absorption measurements have been made on dry samples of poly(dG)-poly(dC) DNA at various relative humidity and temperatures. The water content, controlled by the relative humidity, reduces as temperature increases and reaches a very low value of ∼0.1 wpn above 120°C. This minimum water content is maintained when the samples are brought back to room temperature as long as they are kept at the relative humidity of ∼0%. The molecular vibrations, which characterize the backbone structure as well as the base stacking and pairing, indicate that our samples maintain an A-form double helical structure at all the values of water content. The disorder in the base stacking is observed as the result of the decrease of the water content. Additionally the denaturation appears at high temperatures above 100°C, which reversibly disappears with decreasing temperature.

Original languageEnglish
Article number024009
Journaljournal of the physical society of japan
Volume76
Issue number2
DOIs
Publication statusPublished - 2007 Feb

Keywords

  • DNA
  • Infrared
  • Molecular vibrations
  • Structure
  • Water content

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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