Influence of tip size on single yeast cell imaging using scanning electrochemical microscopy

Kuniaki Nagamine, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The influence of the tip reaction on the substrate generation/tip collection mode SECM imaging of a single yeast cell was investigated by using various sizes of probe electrodes in μm-nm scales because the tip reaction would disturb the concentration profile of intracellular enzyme reactant formed around the cell. GC mode measurements of a single yeast cell were performed using a dual mediator system with lipophilic menadione and hydrophilic ferricyanide. We found downscaling of the tip size enabled quantitative SECM imaging of a single cell in GC mode without disturbing the concentration profile of the mediator formed around the cell by the tip reaction.

Original languageEnglish
Pages (from-to)1168-1174
Number of pages7
JournalElectroanalysis
Volume23
Issue number5
DOIs
Publication statusPublished - 2011 May

Keywords

  • Nanoelectrodes
  • Scanning electrochemical microscopy
  • Single cell analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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