Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film

Sadaaki Yamamoto; Hiroshi Sunami; Masaru Tanaka; Yasutaka Matsuo; Kuniharu Ijiro; Shigeko Yamashita; Yuka Morita; Masatsugu Shimomura

Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film

Sadaaki Yamamoto, Hiroshi Sunami, Masaru Tanaka, Yasutaka Matsuo, Kuniharu Ijiro, Shigeko Yamashita, Yuka Morita, Masatsugu Shimomura

Research output: Contribution to conference › Paper › peer-review

Abstract

The substratum surface morphology plays vital roles in cellular behavior such as adhesion, spreading, migration, proliferation and differentiation We investigated here the structure of a cellular adhesive protein, fibronectin (Fn) adsorbed on both spin-coated films (flat film) and honeycomb pattern films made of poly-ε-caprolactone by using atomic force microscope and confocal laser scanning microscope in relation to the role of the substratum surface morphology in cellular behavior. Although the adsorption saturated at mono-layer adsorption level on both substrates, the structure was inhomogeneous and depend on the surface morphology of substrates: Fn formed fibril-like aggregates on the flat films. Contrary, they adsorbed around the pores on the honeycomb films.

Original language	English
Number of pages	1
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Annual Meeting 2005 - Yokohama, Japan Duration: 2005 May 25 → 2005 May 27

Other

Other	54th SPSJ Annual Meeting 2005
Country	Japan
City	Yokohama
Period	05/5/25 → 05/5/27

Keywords

Cell proliferation
Differentiation
Extracellular matrix
Honeycomb film

ASJC Scopus subject areas

Engineering(all)

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Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film. / Yamamoto, Sadaaki; Sunami, Hiroshi; Tanaka, Masaru; Matsuo, Yasutaka; Ijiro, Kuniharu; Yamashita, Shigeko; Morita, Yuka; Shimomura, Masatsugu.

2005. Paper presented at 54th SPSJ Annual Meeting 2005, Yokohama, Japan.

Research output: Contribution to conference › Paper › peer-review

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abstract = "The substratum surface morphology plays vital roles in cellular behavior such as adhesion, spreading, migration, proliferation and differentiation We investigated here the structure of a cellular adhesive protein, fibronectin (Fn) adsorbed on both spin-coated films (flat film) and honeycomb pattern films made of poly-ε-caprolactone by using atomic force microscope and confocal laser scanning microscope in relation to the role of the substratum surface morphology in cellular behavior. Although the adsorption saturated at mono-layer adsorption level on both substrates, the structure was inhomogeneous and depend on the surface morphology of substrates: Fn formed fibril-like aggregates on the flat films. Contrary, they adsorbed around the pores on the honeycomb films.",

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AU - Yamamoto, Sadaaki

AU - Sunami, Hiroshi

AU - Tanaka, Masaru

AU - Matsuo, Yasutaka

AU - Ijiro, Kuniharu

AU - Yamashita, Shigeko

AU - Morita, Yuka

AU - Shimomura, Masatsugu

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N2 - The substratum surface morphology plays vital roles in cellular behavior such as adhesion, spreading, migration, proliferation and differentiation We investigated here the structure of a cellular adhesive protein, fibronectin (Fn) adsorbed on both spin-coated films (flat film) and honeycomb pattern films made of poly-ε-caprolactone by using atomic force microscope and confocal laser scanning microscope in relation to the role of the substratum surface morphology in cellular behavior. Although the adsorption saturated at mono-layer adsorption level on both substrates, the structure was inhomogeneous and depend on the surface morphology of substrates: Fn formed fibril-like aggregates on the flat films. Contrary, they adsorbed around the pores on the honeycomb films.

AB - The substratum surface morphology plays vital roles in cellular behavior such as adhesion, spreading, migration, proliferation and differentiation We investigated here the structure of a cellular adhesive protein, fibronectin (Fn) adsorbed on both spin-coated films (flat film) and honeycomb pattern films made of poly-ε-caprolactone by using atomic force microscope and confocal laser scanning microscope in relation to the role of the substratum surface morphology in cellular behavior. Although the adsorption saturated at mono-layer adsorption level on both substrates, the structure was inhomogeneous and depend on the surface morphology of substrates: Fn formed fibril-like aggregates on the flat films. Contrary, they adsorbed around the pores on the honeycomb films.

KW - Cell proliferation

KW - Differentiation

KW - Extracellular matrix

KW - Honeycomb film

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T2 - 54th SPSJ Annual Meeting 2005

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Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film

Abstract

Other

Keywords

ASJC Scopus subject areas

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