Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film

Sadaaki Yamamoto, Hiroshi Sunami, Masaru Tanaka, Yasutaka Matsuo, Kuniharu Ijiro, Shigeko Yamashita, Yuka Morita, Masatsugu Shimomura

    Research output: Contribution to conferencePaperpeer-review

    Abstract

    The substratum surface morphology plays vital roles in cellular behavior such as adhesion, spreading, migration, proliferation and differentiation We investigated here the structure of a cellular adhesive protein, fibronectin (Fn) adsorbed on both spin-coated films (flat film) and honeycomb pattern films made of poly-ε-caprolactone by using atomic force microscope and confocal laser scanning microscope in relation to the role of the substratum surface morphology in cellular behavior. Although the adsorption saturated at mono-layer adsorption level on both substrates, the structure was inhomogeneous and depend on the surface morphology of substrates: Fn formed fibril-like aggregates on the flat films. Contrary, they adsorbed around the pores on the honeycomb films.

    Original languageEnglish
    Number of pages1
    Publication statusPublished - 2005 Dec 1
    Event54th SPSJ Annual Meeting 2005 - Yokohama, Japan
    Duration: 2005 May 252005 May 27

    Other

    Other54th SPSJ Annual Meeting 2005
    CountryJapan
    CityYokohama
    Period05/5/2505/5/27

    Keywords

    • Cell proliferation
    • Differentiation
    • Extracellular matrix
    • Honeycomb film

    ASJC Scopus subject areas

    • Engineering(all)

    Fingerprint Dive into the research topics of 'Influence of substratum surface morphologies on the structure of extracellular matrix on a honeycomb film'. Together they form a unique fingerprint.

    Cite this