TY - JOUR
T1 - Influence of pre-freezing conditions of octacalcium phosphate and collagen composite for reproducible appositional bone formation
AU - Yanagisawa, Toshiki
AU - Yasuda, Ayato
AU - Makkonen, Ria I.
AU - Kamakura, Shinji
N1 - Funding Information:
JSPS KAKENHI, Grant/Award Numbers: 18K19891, 16K11741, 16H03159 Funding information
Funding Information:
This study was supported in part by JSPS KAKENHI, grant number 16H03159, 16K11741, and 18K19891.
Publisher Copyright:
© 2020 The Authors. Journal of Biomedical Materials Research Part B: Applied Biomaterials published by Wiley Periodicals, Inc.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Even though conventionally prepared octacalcium phosphate and collagen composite (OCP/Col) has exhibited excellent bone regeneration and has recently been commercialized for treating bone defects, reproducible appositional bone formation with OCP/Col has never been achieved. The present study investigated whether appositional bone formation could be achieved by altering the density of OCP/Col and applying liquid nitrogen during the preparation of OCP/Col. The prepared OCP/Col disks had eight variations and were divided into categories according to four different type of densities (1.0, 1.3, 1.7, and 2.0) of OCP/Col and two different pre-freezing conditions of gas phase (G group: −80°C) and liquid phase (L group: −196°C). These disks were implanted into subperiosteal pockets in rodent calvaria, five samples per each eight variations. Radiomorphometric analysis was conducted at 4 and 12 weeks after implantation, and histological analysis was conducted at 12 weeks after implantation. OCP/Col samples in the L group tended to retain their height and shape and had enhanced appositional bone formation, whereas OCP/Col samples in the G group tended to lose their height and shape and had limited appositional bone formation. The appositional bone formation increased along with growing density of OCP/Col, and L2.0 demonstrated higher appositional bone formation than other samples. These results suggest that the pre-freezing conditions and densities of OCP/Col affect the appositional bone formation.
AB - Even though conventionally prepared octacalcium phosphate and collagen composite (OCP/Col) has exhibited excellent bone regeneration and has recently been commercialized for treating bone defects, reproducible appositional bone formation with OCP/Col has never been achieved. The present study investigated whether appositional bone formation could be achieved by altering the density of OCP/Col and applying liquid nitrogen during the preparation of OCP/Col. The prepared OCP/Col disks had eight variations and were divided into categories according to four different type of densities (1.0, 1.3, 1.7, and 2.0) of OCP/Col and two different pre-freezing conditions of gas phase (G group: −80°C) and liquid phase (L group: −196°C). These disks were implanted into subperiosteal pockets in rodent calvaria, five samples per each eight variations. Radiomorphometric analysis was conducted at 4 and 12 weeks after implantation, and histological analysis was conducted at 12 weeks after implantation. OCP/Col samples in the L group tended to retain their height and shape and had enhanced appositional bone formation, whereas OCP/Col samples in the G group tended to lose their height and shape and had limited appositional bone formation. The appositional bone formation increased along with growing density of OCP/Col, and L2.0 demonstrated higher appositional bone formation than other samples. These results suggest that the pre-freezing conditions and densities of OCP/Col affect the appositional bone formation.
KW - bone augmentation
KW - collagen
KW - liquid nitrogen
KW - multinucleated giant cells
KW - octacalcium phosphate
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U2 - 10.1002/jbm.b.34613
DO - 10.1002/jbm.b.34613
M3 - Article
C2 - 32239797
AN - SCOPUS:85082752601
VL - 108
SP - 2827
EP - 2834
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
SN - 1552-4973
IS - 7
ER -