Influence of osmotic and cationic stresses on high pressure inactivation of Escherichia coli

Toshimi Hasegawa, Takuya Nakamura, Mayumi Hayashi, Miyuki Kido, Masao Hirayama, Toshio Yamaguchi, Akinori Iguchi, Shigeaki Ueno, Toru Shigematsu, Tomoyuki Fujii

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We analyzed high hydrostatic pressure (HP)-induced inactivation of Escherichia coli in the presence of sucrose, NaCl, KCl, or LiCl under 250-400 MPa. Salt stress response (osmotic or cationic) mechanism was demonstrated to be evaluated by HP-induced inactivation kinetics. We selected nine mutant strains from the Keio collection; five strains each lacked one osmoregulatory gene (ΔmzrA, ΔenvZ, ΔompR, ΔompC or ΔompF), and four strains each lacked one cationic stress-response gene (ΔmscS, ΔmscL, ΔchaA or ΔnhaA). The nine mutants exhibited three different patterns of HP-induced inactivation. Of the nine genes, four (mzrA, mscS, nhaA and mscL) were involved in piezotolerance at NaCl concentration of 0.0-0.35 M.

Original languageEnglish
Pages (from-to)292-298
Number of pages7
JournalHigh Pressure Research
Volume33
Issue number2
DOIs
Publication statusPublished - 2013 Jun 1

Keywords

  • Escherichia coli
  • Keio collection
  • high hydrostatic pressure
  • osmotic and cationic stress
  • piezotolerance

ASJC Scopus subject areas

  • Condensed Matter Physics

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