Influence of nitrogen and phosphorus on polyphosphate accumulation in Gigaspora margarita during spore germination

Qing Yao, Ryo Ohtomo, Masanori Saito

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Polyphosphate (polyP) is the form in which phosphorus (P) is transferred from extraradical hyphae into arbuscles in the symbiotic stage of arbuscular mycorrhizal fungi. However, polyP dynamics in the presymbiotic stage are less understood. In this study, we aimed to investigate polyP accumulation in Gigaspora margarita as influenced by nitrogen (N) and/or P supply during germination. Spores of G. margarita were incubated on medium with or without P or N addition. PolyP content in the fungal tissue was monitored using a polyP kinase/luciferase system, and polyP synthetic activity was determined with 32P labeling. The results showed that both N and P were necessary for polyP accumulation in germ tubes. Nitrate increased the polyP content in germ tubes, but ammonium did not. Along with germination, polyP content decreased in spores, but increased in germ tubes. 32P labeling indicated that polyP synthetic activity increased in germ tubes along with germination, but was negligible in spores. Our results suggest that, in the presymbiotic stage of G. margarita, uptake of environmental N and P increases polyP content in germ tubes, and that polyP synthesis occurs mainly therein, leading to polyP accumulation. The possible mechanism of transfer of polyP from spores to hyphae remains to be elucidated.

Original languageEnglish
Pages (from-to)303-311
Number of pages9
JournalPlant and Soil
Volume330
Issue number1
DOIs
Publication statusPublished - 2010 May

Keywords

  • Arbuscular mycorrhizal fungi
  • Nitrogen sources
  • Phosphorus
  • Polyphosphate accumulation
  • Presymbiotic stage

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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