Influence of Molecular Sizes of Cryptococcus neoformans Capsular Polysaccharide on Phagocytosis

Akira Yasuoka, Hiroshi Yamada, Mitsuo Kaku, Hironobu Koga, Shigeru Kohno

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

The role of capsular polysaccharides (CPS) of Cryptococcus neoformans in phagocytosis by murine alveolar macrophages was investigated in four strains of C. neoformans serotype A, YC-11, YC-5, YC-27 and YC-13. Phagocytosis rates increased markedly after adding 10% mouse serum, compared to fetal calf serum. The reverse relation between capsular thickness of C. neoformans and phagocytosis by alveolar macrophages was observed except in YC-27, which had thin capsules and high virulence. The phagocytosis rate in mice serum was 17.3% in YC-11 (capsule thickness 2.8–3.5 μm), 39.8% in YC-5 (capsule size 0.81.5 μm), 20.3% in YC-27 (capsule size 0.6–1.1 μm), and 62.8% in YC-13 (capsule not detected microscopically). The CPS of YC-11, YC-5, and YC-27 analyzed by gel-filtration using CL-2B showed high molecular fractions near the void volume. However, the CPS of YC-13 showed only low molecular fractions. The widely eluted CPS of YC-11 was separated into 3 fractions and each fraction was added in the phagocytosis assay of YC-13. Phagocytosis was markedly suppressed particularly by the addition of a higher molecular fraction. These results suggest that phagocytosis of C. neoformans by alveolar macrophages is influenced by the molecular sizes of the CPS.

Original languageEnglish
Pages (from-to)851-856
Number of pages6
JournalMICROBIOLOGY and IMMUNOLOGY
Volume38
Issue number11
DOIs
Publication statusPublished - 1994

Keywords

  • Antiphagocytic activity
  • Capsular polysaccharide
  • Cryptococcus neoformans
  • Murine
  • alveolar macrophage

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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