Influence of length on cytotoxicity of multi-walled carbon nanotubes against human acute monocytic leukemia cell line THP-1 in vitro and subcutaneous tissue of rats in vivo

Yoshinori Sato, Atsuro Yokoyama, Ken Ichiro Shibata, Yuki Akimoto, Shin Ichi Ogino, Yoshinobu Nodasaka, Takao Kohgo, Kazuchika Tamura, Tsukasa Akasaka, Motohiro Uo, Kenichi Motomiya, Balachandran Jeyadevan, Mikio Ishiguro, Rikizo Hatakeyama, Fumio Watari, Kazuyuki Tohji

Research output: Contribution to journalArticlepeer-review

281 Citations (Scopus)

Abstract

Carbon nanotubes (CNTs) are single- or multi-cylindrical graphene structures that possess diameters of a few nanometers, while the length can be up to a few micrometers. These could have unusual toxicological properties, in that they share intermediate morphological characteristics of both fibers and nanoparticles. To date, no detailed study has been carried out to determine the effect of length on CNT cytotoxicity. In this paper, we investigated the activation of the human acute monocytic leukemia cell line THP-1 in vitro and the response in subcutaneous tissue in vivo to CNTs of different lengths. We used 220 nm and 825 nm-long CNT samples for testing, referred to as "220-CNTs" and "825-CNTs", respectively. 220-CNTs and 825-CNTs induced human monocytes in vitro, although the activity was significantly lower than that of microbial lipopeptide and lipopolysaccharide, and no activity appeared following variation in the length of CNTs. On the other hand, the degree of inflammatory response in subcutaneous tissue in rats around the 220-CNTs was slight in comparison with that around the 825-CNTs. These results indicated that the degree of inflammation around 825-CNTs was stronger than that around 220-CNTs since macrophages could envelop 220-CNTs more readily than 825-CNTs. However, no severe inflammatory response such as necrosis, degeneration or neutrophil infiltration in vivo was observed around both CNTs examined throughout the experimental period.

Original languageEnglish
Pages (from-to)176-182
Number of pages7
JournalMolecular BioSystems
Volume1
Issue number2
DOIs
Publication statusPublished - 2005

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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