Influence of anticoagulants and storage temperatures on blood counts and mitotic index of blood samples collected for cytogenetic biodosimetry

Yohei Fujishima, Syuki Kanahama, Shigeki Hagino, Shiori Natsubori, Hitoshi Saito, Ayaka Azumaya, Kentaro Ariyoshi, Akifumi Nakata, Kosuke Kasai, Kyogo Yamada, Yasushi Mariya, Mitsuaki A. Yoshida, Tomisato Miura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: In order to establish suitable protocols of blood culture to obtain sufficient numbers of metaphases for dicentric chromosome assay (DCA), we have examined the effect of storage temperature, storage time, and anticoagulant type. Materials and methods: Peripheral blood was collected from five healthy donors with lithium heparin and ethylenediaminetetraacetic acid dipotassium salt (EDTA-2K). These samples were irradiated with X-rays at 3 Gy or sham; the samples were further divided into groups that were either stored at room temperature (RT) or 5.2 ± 1.0 °C. After 6, 24, 48, 72, and 168 h of storage, both blood counts and the mitotic index (MI) were analyzed. Results: Heparinized blood samples stored under cold conditions exhibited low white blood cell, lymphocyte, and platelet counts. EDTA-treated blood samples did not show such obvious changes in cell counts. After 6 h of storage, heparinized blood samples stored at RT had MI of 21.5–29.3%. Similar MI was obtained in the EDTA-washed group stored for 6, 24, 48, and 72 h. Conclusions: Our study confirms that heparinized blood samples should be stored at RT to get sufficient metaphases for DCA, and that EDTA blood samples also can be used for blood culture after washing and storage under 5.2 ± 1.0 °C.

Original languageEnglish
Pages (from-to)186-192
Number of pages7
JournalInternational Journal of Radiation Biology
Volume95
Issue number2
DOIs
Publication statusPublished - 2019 Feb 1
Externally publishedYes

Keywords

  • Cytogenetic biodosimetry
  • anticoagulants
  • blood storage
  • mitotic index

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Fujishima, Y., Kanahama, S., Hagino, S., Natsubori, S., Saito, H., Azumaya, A., Ariyoshi, K., Nakata, A., Kasai, K., Yamada, K., Mariya, Y., Yoshida, M. A., & Miura, T. (2019). Influence of anticoagulants and storage temperatures on blood counts and mitotic index of blood samples collected for cytogenetic biodosimetry. International Journal of Radiation Biology, 95(2), 186-192. https://doi.org/10.1080/09553002.2019.1539882