Inflammation-induced lymphangiogenesis in the cornea arises from CD11b-positive macrophages

Kazuichi Maruyama, Masaaki Ii, Claus Cursiefen, David G. Jackson, Hiroshi Keino, Minoru Tomita, Nico Van Rooijen, Hideya Takenaka, Patricia A. D'Amore, Joan Stein-Streilein, Douglas W. Losordo, J. Wayne Streilein

Research output: Contribution to journalArticlepeer-review

565 Citations (Scopus)


In the inflamed cornea, there is a parallel outgrowth of blood and lymphatic vessels into the normally avascular cornea. We tested whether adaptive and/or innate immune cells were actively involved in the genesis of new lymphatic vessels. Our results indicate that innate immune cells (CD11b + macrophages, but not CD11c+ dendritic cells) physically contributed to lymphangiogenesis under pathological conditions and that bone marrow-derived CD11b+ macrophages expressed lymphatic endothelial markers such as LYVE-1 and Prox-1 under inflamed conditions in the corneal stromata of mice. Furthermore, blood vascular endothelial cells that expressed the Tie2 promoter did not contribute to newly formed lymphatic vessels under inflamed conditions. Our in vitro experiments demonstrated that CD11b + macrophages alone were capable of forming tube-like structures that expressed markers of lymphatic endothelium such as LYVE-1 and podoplanin. The novel finding that CD11b+ macrophages are critical for the development of inflammation-dependent lymphangiogenesis in the eye suggests a new mechanism of lymphangiogenesis.

Original languageEnglish
Pages (from-to)2363-2372
Number of pages10
JournalJournal of Clinical Investigation
Issue number9
Publication statusPublished - 2005 Sep
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


Dive into the research topics of 'Inflammation-induced lymphangiogenesis in the cornea arises from CD11b-positive macrophages'. Together they form a unique fingerprint.

Cite this