Inference of gene regulation via miRNAs during ES cell differentiation using MiRaGE method

Masato Yoshizawa, Y. h. Taguchi, Jun Yasuda

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

MicroRNA (miRNA) is a critical regulator of cell growth, differentiation, and development. To identify important miRNAs in a biological process, many bioinformatical tools have been developed. We have developed MiRaGE (MiRNA Ranking by Gene Expression) method to infer the regulation of gene expression by miRNAs from changes of gene expression profiles. The method does not require precedent array normalization. We applied the method to elucidate possibly important miRNAs during embryonic stem (ES) cell differentiation to neuronal cells and we infer that certain miRNAs, including miR-200 family, miR-429, miR-302 family, and miR-17-92 cluster members may be important to the maintenance of undifferentiated status in ES cells.

Original languageEnglish
Pages (from-to)9265-9276
Number of pages12
JournalInternational journal of molecular sciences
Volume12
Issue number12
DOIs
Publication statusPublished - 2011 Dec

Keywords

  • Embryonic stem cell
  • MIRaGE metheod
  • MiRNA

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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