Induction of xylem sap methylglycine by a drought and rewatering treatment and its inhibitory effects on the growth and development of plant organs

Atsushi Oda, Madoka Shimizu, Takeshi Kuroha, Shinobu Satoh

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    In higher plants, the xylem vessels functionally connect the roots with the above-ground organs. The xylem sap transports various organic compounds, such as proteins and amino acids. We examined drought and rewatering-inducible changes in the amino acid composition of root xylem sap collected from Cucurbita maxima roots. The major free amino acids in C. maxima root xylem sap were methylglycine (MeGly; sarcosine) and glutamine (Gln), but MeGly was not detected in the xylem sap of cucumber. MeGly is an intermediate compound in the metabolism of trimethylglycine (TMG; betaine), but its physiological effects in plants are unknown. Drought and rewatering treatment resulted in an increase in the concentration of MeGly in root xylem sap to 2.5 mM. After flowering, the MeGly concentration in the xylem sap dropped significantly, whereas the concentration of Gln decreased only after fruit ripening. One milli molar MeGly inhibited the formation of adventitious roots and their elongation in C. maxima, but glycine, dimethylglycine, or TMG had no effect. Similar effects and the inhibition of stem elongation were observed in shoot cuttings of cucumber and Phaseolus angularis. These observations seem to imply a possible involvement of xylem sap MeGly in the physiological responses of C. maxima plants to drought stress.

    Original languageEnglish
    Pages (from-to)515-523
    Number of pages9
    JournalPhysiologia Plantarum
    Volume124
    Issue number4
    DOIs
    Publication statusPublished - 2005 Aug 1

    ASJC Scopus subject areas

    • Physiology
    • Genetics
    • Plant Science
    • Cell Biology

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