Induction of the nuclear IκB protein IκB-ζ upon stimulation of B cell antigen receptor

Kuniaki Hijioka, Susumu Matsuo, Akiko Eto-Kimura, Koichiro Takeshige, Tatsushi Muta

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The nuclear IκB protein IκB-ζ is barely detectable in resting cells and is induced in macrophages and fibroblasts following stimulation of innate immunity via Toll-like receptors. The induced IκB-ζ associates with nuclear factor (NF)-κB in the nucleus and plays crucial roles in its transcriptional regulation. Here, we examined the induction of IκB-ζ in B lymphocytes, one of the major players in adaptive immunity. Upon crosslinking of the surface immunoglobulin complex, IκB-ζ mRNA was robustly induced in murine B-lymphoma cell line A20 cells. While the crosslinking activated NF-κB and induced its target gene, IκB-α, co-crosslinking of Fcγ receptor IIB to the surface immunoglobulin complex inhibited NF-κB activation and the induction of IκB-ζ and IκB-α, suggesting critical roles for NF-κB in the induction. These results indicate that IκB-ζ is also induced by stimulation of B cell antigen receptor, suggesting that IκB-ζ is involved in the regulation of adaptive immune responses.

Original languageEnglish
Pages (from-to)476-480
Number of pages5
JournalBiochemical and biophysical research communications
Volume356
Issue number2
DOIs
Publication statusPublished - 2007 May 4

Keywords

  • Antigen receptor
  • B lymphocyte
  • Fc receptor
  • ITAM
  • ITIM
  • IκB-ζ
  • NF-κB
  • Post-transcriptional regulation
  • mRNA stabilization

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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