Induction of the histamine-forming enzyme histidine decarboxylase in skeletal muscles by prolonged muscular work: Histological demonstration and mediation by cytokines

Kentaro Ayada, Masahiro Tsuchiya, Hiroyuki Yoneda, Kouji Yamaguchi, Hiroyuki Kumamoto, Keiichi Sasaki, Takeshi Tadano, Makoto Watanabe, Yasuo Endo

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Recent studies suggest that histamine—a regulator of the microcirculation—may play important roles in exercise. We have shown that the histamine-forming enzyme histidine decarboxylase (HDC) is induced in skeletal muscles by prolonged muscular work (PMW). However, histological analysis of such HDC induction is lacking due to appropriate anti-HDC antibodies being unavailable. We also showed that the inflammatory cytokines interleukin (IL)-1 and tumor necrosis factor (TNF)-α can induce HDC, and that PMW increases both IL-1α and IL-1β in skeletal muscles. Here, we examined the effects (a) of PMW on the histological evidence of HDC induction and (b) of IL-1β and TNF-α on HDC activity in skeletal muscles. By immunostaining using a recently introduced commercial polyclonal anti-HDC antibody, we found that cells in the endomysium and around blood vessels, and also some muscle fibers themselves, became HDC-positive after PMW. After PMW, TNF-α, but not IL-1α or IL-1β, was detected in the blood serum. The minimum intravenous dose of IL-1β that would induce HDC activity was about 1/10 that of TNF-α, while in combination they synergistically augmented HDC activity. These results suggest that PMW induces HDC in skeletal muscles, including cells in the endomysium and around blood vessels, and also some muscle fibers themselves, and that IL-1β and TNF-α may cooperatively mediate this induction.

Original languageEnglish
Pages (from-to)1326-1330
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume40
Issue number8
DOIs
Publication statusPublished - 2017

Keywords

  • Exercise
  • Histamine
  • Histidine decarboxylase
  • Interleukin (IL)-1
  • Skeletal muscle
  • Tumor necrosis factor (TNF)

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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