Induction of Th1 immune responses to Japanese cedar pollen allergen (Cry j 1) in mice immunized with Cry j 1 conjugated with CpG oligodeoxynucleotide

Y. Kaburaki, T. Fujimura, K. Kurata, K. Masuda, M. Toda, H. Yasueda, K. Chida, S. Kawarai, M. Sakaguchi

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We determined whether a major Japanese cedar pollen allergen (Cry j 1) conjugated with CpG oligodeoxynucleotide would enhance allergen-specific Th1 responses in mice. Cry j 1 conjugated with CpG (Cry j 1-CpG) induced IL-12 in the spleen cells of naïve mice. Cry j 1-CpG immunization of BALB/c mice suppressed anti-Cry j 1 IgE response and enhanced anti-Cry j 1 IgG2a to subsequent Cry j 1 and alum adjuvant injection. CD4+T cells isolated from the spleens in mice immunized with Cry j 1-CpG produced higher IFN-γ levels than did CD4+T cells obtained from mice as negative controls. Our results suggested that Cry j 1-CpG immunization can induce Cry j 1-specific Th1 immune responses, thereby inhibiting IgE response to the pollen allergen.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume34
Issue number2
DOIs
Publication statusPublished - 2011 Mar 1
Externally publishedYes

Keywords

  • Allergens
  • CpG
  • IgE
  • Pollen

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases

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