TY - JOUR
T1 - Induction of reparative dentin formation by ultrasound-mediated gene delivery of Growth/differentiation factor 11
AU - Nakashima, Misako
AU - Tachibana, Katsuro
AU - Iohara, Koichiro
AU - Ito, Masataka
AU - Ishikawa, Masaki
AU - Akamine, Akifumi
PY - 2003/4/10
Y1 - 2003/4/10
N2 - Bone morphogenetic proteins (BMPs) are morphogens implicated in embryonic and regenerative odontogenic differentiation. Gene therapy has the potential to induce reparative dentin formation for potential pulp capping. We have optimized the gene transfer of Growth/differentiation factor 11 (Gdf11)/Bmp11 plasmid DNA into dental pulp stem cells by sonoporation in viva. Dental pulp tissue treated with plasmid pEGFP or CMV-LacZ in 5-10% Optison (Molecular Biosystems Inc., San Diego, CA) and stimulated by ultrasound (1 MHz, 0.5 W/cm2, 30 sec) showed significant efficiency of gene transfer and high level of protein production selectively in the local region, within 500 μm of the amputated site of the pulp tissue. The Gdf11 cDNA plasmid transferred into dental pulp tissue by sonoporation in vitro, induced the expression of dentin sialoprotein (Dsp), a differentiation marker for odontoblasts. The transfection of Gdf11 by sonoporation stimulated a large amount of reparative dentin formation on the amputated dental pulp in canine teeth in viva. These results suggest the possible use of BMPs using ultrasound-mediated gene therapy for endodontic dental treatment.
AB - Bone morphogenetic proteins (BMPs) are morphogens implicated in embryonic and regenerative odontogenic differentiation. Gene therapy has the potential to induce reparative dentin formation for potential pulp capping. We have optimized the gene transfer of Growth/differentiation factor 11 (Gdf11)/Bmp11 plasmid DNA into dental pulp stem cells by sonoporation in viva. Dental pulp tissue treated with plasmid pEGFP or CMV-LacZ in 5-10% Optison (Molecular Biosystems Inc., San Diego, CA) and stimulated by ultrasound (1 MHz, 0.5 W/cm2, 30 sec) showed significant efficiency of gene transfer and high level of protein production selectively in the local region, within 500 μm of the amputated site of the pulp tissue. The Gdf11 cDNA plasmid transferred into dental pulp tissue by sonoporation in vitro, induced the expression of dentin sialoprotein (Dsp), a differentiation marker for odontoblasts. The transfection of Gdf11 by sonoporation stimulated a large amount of reparative dentin formation on the amputated dental pulp in canine teeth in viva. These results suggest the possible use of BMPs using ultrasound-mediated gene therapy for endodontic dental treatment.
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U2 - 10.1089/104303403764539369
DO - 10.1089/104303403764539369
M3 - Article
C2 - 12718768
AN - SCOPUS:0037431212
VL - 14
SP - 591
EP - 597
JO - Human Gene Therapy
JF - Human Gene Therapy
SN - 1043-0342
IS - 6
ER -