Induction of non-apoptotic cell death by morphinone in human promyelocytic leukemia HL-60 cells

Risa Takeuchi, Hiroshi Hoshijima, Hiroshi Nagasaka, Shahead Ali Chowdhury, Hirotaka Kikuchi, Yumiko Kanda, Shiro Kunii, Masami Kawase, Hiroshi Sakagami

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

As previously suggested, codeinone (oxidation product of codeine) induces non-apoptotic cell death, characterized by marginal caspase activation and the lack of DNA fragmentation in HL-60 human promyelocytic leukemia cells, which was inhibited by N-acetyl-L-cysteine. Whether, morphinone, an oxidative metabolite of morphine, also induced a similar type of cell death in HL-60 cells was investigated. Morphinone showed slightly higher cytotoxic activity against human tumor cell lines (oral squamous cell carcinoma HSC-2, HSC-3, HSC-4, NA, Ca9-22, promyelocytic leukemia HL-60, cervical carcinoma HeLa) than against normal oral human cells (gingival fibroblast HGF, pulp cells HPC, periodontal ligament fibroblast HPLF). Morphinone also induced an almost undetectable level of internucleosomal DNA fragmentation in the HL-60 cells. Morphinone did not activate caspase-8 or -9 in these cells. Morphinone dose-dependently activated caspase-3 in both HL-60 and HSC-2 cell lines, but to a much lesser extent than actinomycin D. Electron microscopy demonstrated that morphinone induced mitochondrial shrinkage, vacuolization and production of autophagosome and the loss of cell surface microvilli, without destruction of cell surface and nuclear membranes in the HL-60 cells. The autophagy inhibitor 3-methyladenine (0.3-10 mM) slightly inhibited the morphinone-induced cytotoxicity, when corrected for its own cytotoxicity. These data suggest that morphinone induces non-apoptotic cell death in HL-60 cells.

Original languageEnglish
Pages (from-to)3343-3348
Number of pages6
JournalAnticancer research
Volume26
Issue number5 A
Publication statusPublished - 2006 Sep 1
Externally publishedYes

Keywords

  • Anticancer drugs
  • Mitochondria
  • Morphinone
  • Non-apoptotic cell death
  • Tumor-specificity
  • α,β-unsaturated ketones

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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