Induction of nodule-like structures on oilseed rape by inoculation with rhizobia in the presence of helper bacteria

Shoichiro Akao, Makie Kokubun, Makie Kokubun, Perigio B. Francisco, Shoichiro Akao, Makie Kokubun, Perigio B. Francisco, Shoichiro Akao

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Nodule-like structures on the roots of seedlings of oilseed rape (Brassica campestris L. subsp. napus) cv. CO were induced by Bradyrhizobium sp. strain CP283 or A2P5in the presence of Erwinia carotovora MAFF-301393, a bacterium that produces the cell wall-degrading enzymes cellulase and pectinase. E. carotovora was most effective at an inoculation density of 2.0 × 108cells plant-1. Lower densities decreased the rate of nodule-like structure formation, while higher densities caused plant death. The induced nodule-like structures were either spherical or elongated. Histological sections of the structures showed a distinct central vascular cylinder and a surrounding swollen region composed entirely of cortical cells. The highest rate of nodule-like structure formation (33.3%) was achieved by combined inoculation of B. sp. strain CP283 and E. carotovora.

    Original languageEnglish
    Pages (from-to)313-320
    Number of pages8
    JournalSoil Science and Plant Nutrition
    Volume41
    Issue number2
    DOIs
    Publication statusPublished - 1995 Jan 1

    Keywords

    • Erwinia carotovora
    • Nodule-like structure
    • Oilseed rape (Brassica napus)

    ASJC Scopus subject areas

    • Soil Science
    • Plant Science

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  • Cite this

    Akao, S., Kokubun, M., Kokubun, M., Francisco, P. B., Akao, S., Kokubun, M., Francisco, P. B., & Akao, S. (1995). Induction of nodule-like structures on oilseed rape by inoculation with rhizobia in the presence of helper bacteria. Soil Science and Plant Nutrition, 41(2), 313-320. https://doi.org/10.1080/00380768.1995.10419588