TY - JOUR
T1 - Induction of MITF expression in human cholangiocarcinoma cells and hepatocellular carcinoma cells by cyclopamine, an inhibitor of the Hedgehog signaling
AU - Samatiwat, Papavee
AU - Takeda, Kazuhisa
AU - Satarug, Soisungwan
AU - Ohba, Koji
AU - Kukongviriyapan, Veerapol
AU - Shibahara, Shigeki
N1 - Funding Information:
P. Samatiwat received the Royal Golden Jubilee Ph.D. scholarship from the Commission for Higher Education, Thailand Ministry of Education. S. Satarug (L-14568) received Research Fellowship from the Japan Society for Promotion of Science (JSPS). We are grateful to Biomedical Research Core of Tohoku University Graduate School of Medicine for allowing us to use various facilities.
Publisher Copyright:
© 2016 Elsevier Inc. All rights reserved.
PY - 2016/1/29
Y1 - 2016/1/29
N2 - Microphthalmia-associated transcription factor (MITF) is a key regulator of differentiation of melanocytes and retinal pigment epithelial cells, but it also has functions in non-pigment cells. MITF consists of multiple isoforms, including widely expressed MITF-A and MITF-H. In the present study, we explored the potential role played by the Hedgehog signaling on MITF expression in two common types of primary liver cancer, using human cholangiocarcinoma cell lines, the KKU-100 and HuCCT1, along with the HepG2 human hepatocellular carcinoma cell line. Importantly, cholangiocarcinoma is characterized by the activated Hedgehog signaling. Here we show that MITF-A mRNA is predominantly expressed in all three human liver cancer cell lines examined. Moreover, cyclopamine, an inhibitor of the Hedgehog signalling, increased the expression levels of MITF proteins in HuCCT1 and HepG2 cells, but not in KKU-100 cells, suggesting that MITF expression may be down-regulated in some liver cancer cases.
AB - Microphthalmia-associated transcription factor (MITF) is a key regulator of differentiation of melanocytes and retinal pigment epithelial cells, but it also has functions in non-pigment cells. MITF consists of multiple isoforms, including widely expressed MITF-A and MITF-H. In the present study, we explored the potential role played by the Hedgehog signaling on MITF expression in two common types of primary liver cancer, using human cholangiocarcinoma cell lines, the KKU-100 and HuCCT1, along with the HepG2 human hepatocellular carcinoma cell line. Importantly, cholangiocarcinoma is characterized by the activated Hedgehog signaling. Here we show that MITF-A mRNA is predominantly expressed in all three human liver cancer cell lines examined. Moreover, cyclopamine, an inhibitor of the Hedgehog signalling, increased the expression levels of MITF proteins in HuCCT1 and HepG2 cells, but not in KKU-100 cells, suggesting that MITF expression may be down-regulated in some liver cancer cases.
KW - Bile duct
KW - Cholangiocyte
KW - Cyclopamine
KW - Hedgehog
KW - Hepatocyte
KW - MITF
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U2 - 10.1016/j.bbrc.2016.01.010
DO - 10.1016/j.bbrc.2016.01.010
M3 - Article
C2 - 26773496
AN - SCOPUS:84956596434
VL - 470
SP - 144
EP - 149
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -
