TY - JOUR
T1 - Induction of midkine expression in reactive astrocytes following rat transient forebrain ischemia
AU - Mochizuki, Ruriko
AU - Takeda, Atsushi
AU - Sato, Nobuyuki
AU - Kimpara, Teiko
AU - Onodera, Hiroshi
AU - Itoyama, Yasuto
AU - Muramatsu, Takashi
PY - 1998/1
Y1 - 1998/1
N2 - Midkine (MK), a retinoic acid-responsive gene product, is a 13-kDa heparin-binding protein with neurotrophic activity. Previous studies demonstrated the expression of MK in embryonal and neonatal brains and its potent neurotrophic activities in vitro. Data concerning its role in the mature central nervous system, however, are still limited. We examined the changes of MK expression in the adult rat brain following transient forebrain ischemia, by Northern blot, in situ hybridization and immunohistochemical analyses. In the control brain, MK mRNA was expressed in the cortical and hippocampal neurons. Following the ischemia, up-regulation of MK mRNA and a corresponding increase of its protein products were found in the hippocampal CA1 subfield. The maximal expression was demonstrated on day 4 after the insult. The cells expressing MK were distributed around the depleted CA1 pyramidal cells and identified as reactive astrocytes by double immunostaining. These data suggest that MK may be an insult-induced molecule which participates in the reparative processes following neuronal injury.
AB - Midkine (MK), a retinoic acid-responsive gene product, is a 13-kDa heparin-binding protein with neurotrophic activity. Previous studies demonstrated the expression of MK in embryonal and neonatal brains and its potent neurotrophic activities in vitro. Data concerning its role in the mature central nervous system, however, are still limited. We examined the changes of MK expression in the adult rat brain following transient forebrain ischemia, by Northern blot, in situ hybridization and immunohistochemical analyses. In the control brain, MK mRNA was expressed in the cortical and hippocampal neurons. Following the ischemia, up-regulation of MK mRNA and a corresponding increase of its protein products were found in the hippocampal CA1 subfield. The maximal expression was demonstrated on day 4 after the insult. The cells expressing MK were distributed around the depleted CA1 pyramidal cells and identified as reactive astrocytes by double immunostaining. These data suggest that MK may be an insult-induced molecule which participates in the reparative processes following neuronal injury.
KW - Brain insult
KW - CA1
KW - Heparin-binding growth-associated molecule
KW - Heparin-binding protein
KW - Neurotrophic factor
KW - Reparative process
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U2 - 10.1006/exnr.1997.6687
DO - 10.1006/exnr.1997.6687
M3 - Article
C2 - 9454616
AN - SCOPUS:0031936041
VL - 149
SP - 73
EP - 78
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 1
ER -