Induction of lipocalin-type prostaglandin D synthase in mouse heart under hypoxemia

Feng Han, Kazuhisa Takeda, Kazunobu Ishikawa, Masao Ono, Fumiko Date, Satoru Yokoyama, Kazumichi Furuyama, Yotaro Shinozawa, Yoshihiro Urade, Shigeki Shibahara

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Hypoxemia is a common manifestation of various disorders and generates pressure overload to the heart. Here we analyzed the expression of lipocalin-type prostaglandin D synthase (L-PGDS) in the heart of C57BL/6 mice kept under normobaric hypoxia (10% O2) that generates hemodynamic stress. Northern and Western blot analyses revealed that the expression levels of L-PGDS mRNA and protein were significantly increased (>twofold) after 14 days of hypoxia, compared to the mice kept under normoxia. Immunohistochemical analysis indicated that L-PGDS was increased in the myocardium of auricles and ventricles and the pulmonary venous myocardium at 28 days of hypoxia. Moreover, using C57BL/6 mice lacking heme oxygenase-2 (HO-2-/-), a model of chronic hypoxemia, we showed that the expression level of L-PGDS protein was twofold higher in the heart than that of wild-type mouse. L-PGDS expression is induced in the myocardium under hypoxemia, which may reflect the adaptation to the hemodynamic stress.

Original languageEnglish
Pages (from-to)449-453
Number of pages5
JournalBiochemical and biophysical research communications
Volume385
Issue number3
DOIs
Publication statusPublished - 2009 Jul 31

Keywords

  • Heart
  • Heme oxygenase
  • Hypoxia
  • Lung
  • Prostaglandin D
  • Prostaglandin D synthase
  • Pulmonary venous myocardium

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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