Induction of glycophorin gene expression in cultured murine erythroleukemia cells

Yasuhisa Matsui, Shunji Natori, Masuo Obinata

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

In order to identify the mRNA for mouse glycophorin, mRNA was isolated from immature erythroid cells obtained from the spleens of anemic mice, translated in vitro in mRNA-dependent rabbit reticulocyte lysate, and then immunoprecipitated with a specific antiserum. Glycophorin mRNA was shown to be present only in erythroid cells. In immunofluorescent and in vitro translation studies, it was shown that glycophorin mRNA is absent in uninduced murine erythroleukemia (MEL) cells, but is induced in dimethylsulfoxide-treated differentiating cells.

Original languageEnglish
Pages (from-to)268-274
Number of pages7
JournalDifferentiation
Volume29
Issue number3
DOIs
Publication statusPublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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