Induction of erythroid-specific gene expression in lymphoid cells

T. Chiba, Y. Nagata, A. Kishi, K. Sakamaki, A. Miyajima, M. Yamamoto, J. D. Engel, K. Todokoro

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Erythropoietin (Epo) is a cytokine which specifically regulates differentiation and proliferation of erythroid progenitor cells. We report here that Epo receptor expressed in interleukin 3-dependent lymphoid Ba/F3 cells transmits both differentiation and growth signals. Epo stimulation of these cells leads to activation of transcription and/or translation of the erythroid-specific transcription factors GATA-1 and SCL, followed by the accumulation of both α- and β-globin chains. These results suggest that expression and activation of the Epo receptor regulates erythroid-specific gene expression and might play a role in determining a cell lineage in vivo and that GATA-1 and SCL may exert their effects after Epo binds to its receptor. It was further found that chimeric receptors composed of extracellular domains of Epo receptor and cytoplasmic domains of interleukin 2 or interleukin 3 receptors could also induce erythroid-specific gene expression in Ba/F3 cells. Taking these data together with previous observations, we conclude that interaction of the extracellular domains of the Epo receptor with other membrane components is essential for transmission of both the erythroid differentiation and the growth signals.

Original languageEnglish
Pages (from-to)11593-11597
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number24
DOIs
Publication statusPublished - 1993 Dec 29

Keywords

  • differentiation
  • erythropoietin receptor
  • globin
  • transcription factors GATA-1 and SCL

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Induction of erythroid-specific gene expression in lymphoid cells'. Together they form a unique fingerprint.

  • Cite this