Induction of erythoid-specific genes by overexpression of GATA-2 in K562 cells

Hideo Harigae, Yoko Okitsu, Hisayuki Yokoyama, Tohru Fujiwara, Mitsue Inomata, Shinichiro Takahashi, Naoko Minegishi, Mitsuo Kaku, Takeshi Sasaki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

GATA transcription factors have been shown to play important roles in hematopoiesis. GATA-2 is expressed in stem and progenitor cells, and has been speculated to control the proliferation and maintain the immaturity of these cells. To examine whether the function of GATA-2 is changeable according to the differentiation stage, we established GATA-2 overexpressing subclones of K562, which is a leukemic cell line committed to the erythroid lineage. Via an increase in the GATA-2 expression level, the expression levels of erythroid-specific genes including α-, β-, and γ-globin were increased compared to control cells, while the expression level of GATA-1 was unchanged. Expression of the transferrin receptor was also increased in GATA-2 overexpressing K562 cells when examined by flow cytometry. In addition, the heme content of GATA-2 overexpressing K562 cells was more than 2 times higher than control cells. Chromatin immunoprecipitation analysis showed that GATA-2 protein binding to the GATA element in α-globin LCR was increased in GATA-2 overexpressing K562 cells. These findings suggest that GATA-2 could induce erythroid-specific genes without competition with GATA-1 when expressed in erythroid-committed cells, and thus further suggest that temporal and spatial regulation may be important for displaying specific functions of GATA-2.

Original languageEnglish
Pages (from-to)38-42
Number of pages5
JournalInternational journal of hematology
Volume84
Issue number1
DOIs
Publication statusPublished - 2006 Jul

Keywords

  • Erythroid-specific genes
  • GATA-2
  • K562 cells

ASJC Scopus subject areas

  • Hematology

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