Induction of enamel matrix protein expression in an ameloblast cell line co-cultured with a mesenchymal cell line in vitro

Asako Matsumoto, Hidemitsu Harada, Masahiro Saito, Akiyoshi Taniguchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Interactions between epithelium and mesenchyme are important for organ and tissue development. In this study, in order to mimic interactions between epithelium and mesenchyme during native tooth development, we constructed three-dimensional culture systems in vitro using a collagen membrane. Two types of collagen membrane-based in vitro culture systems were constructed in which dental epithelial and dental follicle cell lines were cultured. One co-culture method involved inoculation of one cell line into one side of the collagen membrane, and the other cell line into the opposite side of the membrane (sandwich co-culture). As a control, the second method involved culture of one of the cell lines on a culture dish and the second cell line on a collagen membrane, facing away from the first cell line (separate co-culture). The HAT- 7 cells were also grown as a monolayer culture on collagen. Ameloblast differentiation in these cultures was investigated by analysis of the mRNA and/or protein expression of ameloblastin and amelogenin. Our results suggest that interaction of epithelial and mesenchymal cells via the extracellular matrix is important for tooth differentiation in vitro. Our culture system should be a useful method for investigation of epithelial-mesenchymal interactions.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume47
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1
Externally publishedYes

Keywords

  • Ameloblastin
  • Amelogenin
  • Co-culture
  • ECM
  • Tooth differentiation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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