Induction of Apg-1, a member of the heat shock protein 110 family, following transient forebrain ischemia in the rat brain

Jing Hui Xue, Hidenao Fukuyama, Kohsuke Nonoguchi, Yoshiyuki Kaneko, Tsuneo Kido, Manabu Fukumoto, Yasuhisa Fujibayashi, Katsuhiko Itoh, Jun Fujita

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31 Citations (Scopus)


Apg-1(Osp94) and apg-2 belong to the heat shock protein (hsp)110 family. In mouse somatic cells the apg-1 and hsp105/110 transcripts are inducible by a 32°C to 39°C heat shock, while apg-2 is not heat-inducible. Since ischemia is known to induce expression of hsp70, its effect on expression of apg-1 was assessed by using the 20-min forebrain ischemia model of the rat. In the cerebral cortex, Northern blot analysis and in situ hybridization histochemistry demonstrated an increased expression in neuronal cells of apg-1 transcripts 3 h after the onset of reperfusion, with a peak at 12 h, followed by a decline. In the hippocampus, the level was increased at 3 h, remained constant until 24 h, and then declined. Transcript levels of apg-2 as well as hspl05 were also increased under the present conditions, indicating that the expression of apg-2 was differentially regulated in response to heat and ischemic stresses. The induction kinetics of hsp105, but neither apg-2 nor hsp70, were identical to those of apg-1. These results demonstrated that brain ischemia/reperfusion induced expression of each member of the hsp110 family, although the regulatory mechanisms may not be the same. They also suggest a significant role of apg-1 in both the ischemic-and heat-stress responses and in the normal functioning of the non-stressed neuronal cells.

Original languageEnglish
Pages (from-to)796-801
Number of pages6
JournalBiochemical and biophysical research communications
Issue number3
Publication statusPublished - 1998 Jun 29
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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