Induction of activated caspase-3-immunoreactivity and apoptosis in the trigeminal ganglion neurons by neonatal peripheral nerve injury

Tomosada Sugimoto, Haiwei Jin, Masako Fijita, Tomohiro Fukunaga, Noriyuki Nagaoka, Tomoichiro Yamaai, Hiroyuki Ichikawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Immunohistochemistry for activated caspase-3 and terminal deoxynucleotidyl transferease-mediated dUTP-biotin nick end labeling (TUNEL) was performed on the trigeminal ganglion after infraorbital nerve transection in newborn rats. The injury induced caspase-3-immunoreactivity and DNA fragmentation in neuronal cell bodies in the maxillary division of the ganglion ipsilateral to the injury. Starting at 16 h post-injury the immunoreactive and TUNEL-positive neurons increased and reached the peak at 24 h (7.9% and 8.9%, respectively). Thereafter they decreased and returned to the normal control level (1%) by 72 h. A double staining procedure revealed coexpression of caspase-3-immunoreactivity and DNA fragmentation. 75.5% (114/151) of TUNEL-positive neurons expressed the immunoreactivity, while 84.4% (114/135) of immunoreactive neurons exhibited DNA fragmentation signal. These results suggest that caspase-3 plays an important role in apoptotic elimination of neonatally axotomized rodent primary neurons.

Original languageEnglish
Pages (from-to)238-243
Number of pages6
JournalBrain research
Volume1017
Issue number1-2
DOIs
Publication statusPublished - 2004 Aug 13
Externally publishedYes

Keywords

  • Apoptosis
  • Caspase-3
  • Development and regeneration
  • Nerve injury
  • Neuronal death
  • Newborn rat
  • TUNEL
  • Trigeminal ganglion

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Induction of activated caspase-3-immunoreactivity and apoptosis in the trigeminal ganglion neurons by neonatal peripheral nerve injury'. Together they form a unique fingerprint.

Cite this