Inducible production of recombinant xylose isomerase by escherichia coli in fed-batch culture

Tomoyasu Kawabe, Takayuki Ohshima, Nobuyuki Uozumi, Shinji Iijima, Takeshi Kobayashi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Escherichia coli JM105 harboring an expression plasmid which bears the xylose isomerase gene controlled by tac promoter was cultivated under different conditions in order to find an optimal fermentation condition. By shake-flask cultures it was found that low concentration of organic nitrogen sources such as yeast extract or casamino acids was essential for efficient production of xylose isomerase. By fed-batch culture in a jar-fermentor, we obtained very high cell concentration. Glucose did not hamper gene expression as much as glycerol. Excess addition of yeast extract lowered the specific activity of the gene product. Although the specific activity of the enzyme in high-concentration culture was a half that in batch culture, the amount of enzyme produced by unit volume in a representative high-concentration culture was more than 33-fold that by batch culture.

Original languageEnglish
Pages (from-to)702-708
Number of pages7
JournalJOURNAL of CHEMICAL ENGINEERING of JAPAN
Volume25
Issue number6
DOIs
Publication statusPublished - 1992 Jan 1
Externally publishedYes

Keywords

  • Biochemical Engineering
  • Escherichia coli
  • Gene Expression
  • Recombinant DNA
  • Tac Promoter
  • Xylose Isomerase

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Fingerprint Dive into the research topics of 'Inducible production of recombinant xylose isomerase by escherichia coli in fed-batch culture'. Together they form a unique fingerprint.

  • Cite this