TY - JOUR
T1 - Inducible nitric oxide synthase following hypoxia in rat cultured glial cells
AU - Kawase, Makoto
AU - Kinouchi, Hiroyuki
AU - Kato, Ichiro
AU - Akabane, Atsuya
AU - Kondo, Takeo
AU - Arai, Shouichi
AU - Fujimura, Miki
AU - Okamoto, Hiroshi
AU - Yoshimoto, Takashi
PY - 1996/11/4
Y1 - 1996/11/4
N2 - Nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) exerts inhibitory and cytotoxic effects on various cells including neuronal cells. In the present study, we examined the ability of rat glial cells to produce NO following hypoxia/reoxygenation in vitro by measuring nitrite. The levels of nitrite produced in the cultured media of glial cells significantly increased after 12-h hypoxia but not after 0- and 6-h hypoxia. The NOS inhibitor, N(G)-monomethyl-L-arginine, decreased hypoxia-induced nitrite formation. In glial cells after hypoxia/reoxygenation, the iNOS mRNA and protein expressions were detected by reverse-transcription polymerase chain reaction and by immunocytochemical analysis, respectively. The present study provides the first evidence that hypoxia induces NO production from glial cells. This hypoxia-induced, glial cell-derived NO may play a critical role in the pathogenesis of cerebral ischemia in vivo.
AB - Nitric oxide (NO) produced by inducible nitric oxide synthase (iNOS) exerts inhibitory and cytotoxic effects on various cells including neuronal cells. In the present study, we examined the ability of rat glial cells to produce NO following hypoxia/reoxygenation in vitro by measuring nitrite. The levels of nitrite produced in the cultured media of glial cells significantly increased after 12-h hypoxia but not after 0- and 6-h hypoxia. The NOS inhibitor, N(G)-monomethyl-L-arginine, decreased hypoxia-induced nitrite formation. In glial cells after hypoxia/reoxygenation, the iNOS mRNA and protein expressions were detected by reverse-transcription polymerase chain reaction and by immunocytochemical analysis, respectively. The present study provides the first evidence that hypoxia induces NO production from glial cells. This hypoxia-induced, glial cell-derived NO may play a critical role in the pathogenesis of cerebral ischemia in vivo.
KW - glial cell
KW - hypoxia
KW - inducible nitric oxide synthase
KW - interferon-γ
KW - interleukin-1β
KW - nitric oxide
KW - nitric oxide synthase
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U2 - 10.1016/S0006-8993(96)00924-9
DO - 10.1016/S0006-8993(96)00924-9
M3 - Article
C2 - 8955528
AN - SCOPUS:0030569336
VL - 738
SP - 319
EP - 322
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 2
ER -