Induced sensitization to nickel in guinea pigs immunized with mycobacteria by injection of purified protein derivative with nickel

H. Kitaura, N. Nakao, N. Yoshida, T. Yamada

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Nickel has been reported to be one of the most common causes of allergic contact dermatitis. Despite the fact that nickel is a frequent sensitizer in humans, establishing animal models for nickel allergy has met with considerable difficulties. In clinical cases, allergic contact hypersensitivity to nickel develops much more readily in inflamed skin than normal skin. In this study, we tried to induce nickel sensitization when inflammation has been evoked in guinea pigs immunized with mycobacteria followed by co-administration of a mycobacterial component with nickel. We first examined the delayed-type hypersensitivity (DTH) reaction of mycobacterial components such as the cell wall, cell membrane, 70S ribosomal fraction, cytoplasm, tuberculin purified protein derivative (PPD), RNA and DNA from Mycobacterium bovis BCG in guinea pigs immunized with live M. bovis BCG or heat killed M. tuberculosis. When PPD was used, the hypersensitivity reaction was strongest. Next, we tested whether PPD with nickel could induce nickel sensitivity in guinea pigs immunized with mycobacteria. Strong sensitization to nickel was achieved by injecting PPD with nickel. However, if too large an amount of PPD or nickel salts was used, sensitization to nickel decreased. In this way, sensitization of nickel developed much more easily in guinea pigs immunized with mycobacteria by injection of an appropriate amount of nickel at the inflammation site induced by a suitable amount of PPD.

Original languageEnglish
Pages (from-to)101-108
Number of pages8
JournalNew Microbiologica
Volume26
Issue number1
Publication statusPublished - 2003 Jan
Externally publishedYes

Keywords

  • Guinea pig
  • Mycobacteria
  • Nickel
  • PPD

ASJC Scopus subject areas

  • Microbiology (medical)

Fingerprint

Dive into the research topics of 'Induced sensitization to nickel in guinea pigs immunized with mycobacteria by injection of purified protein derivative with nickel'. Together they form a unique fingerprint.

Cite this