TY - JOUR
T1 - Induced pluripotent stem cells with six reprogramming factors from prairie vole, which is an animal model for social behaviors
AU - Katayama, Masafumi
AU - Hirayama, Takashi
AU - Horie, Kengo
AU - Kiyono, Tohru
AU - Donai, Kenichiro
AU - Takeda, Satoru
AU - Nishimori, Katsuhiko
AU - Fukuda, Tomokazu
N1 - Funding Information:
We thank Dr. Kenji Sakimura (Niigata University) and Dr. Shizu Hidema (Tohoku University) for helpful discussions. We thank Dr. Larry J. Young (Yerkes National Primate Research Center, Department of Psychiatry, Emory University) for providing prairie voles. This work was supported by JSPS KAKENHI, grant No. 13J04781. The authors declare no conflicts of interest.
Publisher Copyright:
© 2016 Cognizant, LLC.
PY - 2016
Y1 - 2016
N2 - Prairie voles show strong pair bonding with their mating partners, and they demonstrate parental behavior toward their infants, indicating that the prairie vole is a unique animal model for analysis of molecular mechanisms of social behavior. Until a recent study, the signaling pathway of oxytocin was thought to be critical for the social behavior of prairie voles, but neuron-specific functional research may be necessary to identify the molecular mechanisms of social behavior. Prairie vole pluripotent stem cells of high quality are essential to elucidate the molecular mechanisms of social behaviors. Generation of high-quality induced pluripotent stem cells (iPSCs) would help to establish a genetically modified prairie vole, including knockout and knock-in models, based on the pluripotency of iPSCs. Thus, we attempted to establish high-quality prairie vole-derived iPSCs (pv-iPSCs) in this study. We constructed a polycistronic reprogramming vector, which included six reprograming factors (Oct3/4, Sox2, Klf4, c-myc, Lin28, and Nanog). Furthermore, we evaluated the effect of six reprogramming factors, which included Oct3/4 with the transactivation domain (TAD) of MyoD. Implantation of the pv-iPSCs into immunodeficient mice caused a teratoma with three germ layers. Furthermore, the established pv-iPSCs tested positive for stem cell markers, including alkaline phosphatase activity (ALP), stage-specific embryonic antigen (SSEA)-1, and dependence on leukemia inhibitory factor (LIF). Our data indicate that our newly established pv-iPSCs may be a useful tool for genetic analysis of social behavior.
AB - Prairie voles show strong pair bonding with their mating partners, and they demonstrate parental behavior toward their infants, indicating that the prairie vole is a unique animal model for analysis of molecular mechanisms of social behavior. Until a recent study, the signaling pathway of oxytocin was thought to be critical for the social behavior of prairie voles, but neuron-specific functional research may be necessary to identify the molecular mechanisms of social behavior. Prairie vole pluripotent stem cells of high quality are essential to elucidate the molecular mechanisms of social behaviors. Generation of high-quality induced pluripotent stem cells (iPSCs) would help to establish a genetically modified prairie vole, including knockout and knock-in models, based on the pluripotency of iPSCs. Thus, we attempted to establish high-quality prairie vole-derived iPSCs (pv-iPSCs) in this study. We constructed a polycistronic reprogramming vector, which included six reprograming factors (Oct3/4, Sox2, Klf4, c-myc, Lin28, and Nanog). Furthermore, we evaluated the effect of six reprogramming factors, which included Oct3/4 with the transactivation domain (TAD) of MyoD. Implantation of the pv-iPSCs into immunodeficient mice caused a teratoma with three germ layers. Furthermore, the established pv-iPSCs tested positive for stem cell markers, including alkaline phosphatase activity (ALP), stage-specific embryonic antigen (SSEA)-1, and dependence on leukemia inhibitory factor (LIF). Our data indicate that our newly established pv-iPSCs may be a useful tool for genetic analysis of social behavior.
KW - Animal model
KW - Induced pluripotent stem cells (iPSCs)
KW - Prairie vole
KW - Social behavior
KW - Transposon
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U2 - 10.3727/096368916X690502
DO - 10.3727/096368916X690502
M3 - Article
C2 - 26777120
AN - SCOPUS:84964309729
VL - 25
SP - 783
EP - 796
JO - Cell Transplantation
JF - Cell Transplantation
SN - 0963-6897
IS - 5
M1 - 14
ER -