Incubation type planar patch clamp biosensor: Basic performances

Tsuneo Urisu; Hidetaka Uno; Zhi Hong Wang; Senthil Kumar Obuliraj; Noriko Takada; Masaki Aoyama; Mitsukazu Suzui; Toshifumi Asano; Toru Ishizuka; Hiromu Yawo

Incubation type planar patch clamp biosensor: Basic performances

Tsuneo Urisu, Hidetaka Uno, Zhi Hong Wang, Senthil Kumar Obuliraj, Noriko Takada, Masaki Aoyama, Mitsukazu Suzui, Toshifumi Asano, Toru Ishizuka, Hiromu Yawo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

The biosensors based on the incubation type planar patch clamp method was developed and the basic properties were investigated. Usefulness of light-gated ionchannel method on the performance of the device was confirmed. The excess current noise and the thermal noise due to the micropore resistance and the seal resistance were the main sources of the noise, and the noise level of the developed biosensor was 7 pA at the 1 kHz low pass filter. This value is slightly larger than the single ionchannel current level (∼4pA) of TRPV1. We consider that the developed device has a sufficient performance for the whole cell measurements, and extremely suitable for the high throughput screening application with neural network, in which incubation function is essentially necessary.

Original language	English
Title of host publication	BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices
Pages	143-148
Number of pages	6
Publication status	Published - 2012
Event	International Conference on Biomedical Electronics and Devices, BIODEVICES 2012 - Vilamoura, Algarve, Portugal Duration: 2012 Feb 1 → 2012 Feb 4

Publication series

Name	BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices

Other

Other	International Conference on Biomedical Electronics and Devices, BIODEVICES 2012
Country/Territory	Portugal
City	Vilamoura, Algarve
Period	12/2/1 → 12/2/4

Keywords

Biosensor
HEK293
Ionchannel
PMMA
Planar patch clamp
TRPV1

ASJC Scopus subject areas

Biomedical Engineering
Electrical and Electronic Engineering

Cite this

Urisu, T., Uno, H., Wang, Z. H., Obuliraj, S. K., Takada, N., Aoyama, M., Suzui, M., Asano, T., Ishizuka, T., & Yawo, H. (2012). Incubation type planar patch clamp biosensor: Basic performances. In BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices (pp. 143-148). (BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices).

Incubation type planar patch clamp biosensor : Basic performances. / Urisu, Tsuneo; Uno, Hidetaka; Wang, Zhi Hong; Obuliraj, Senthil Kumar; Takada, Noriko; Aoyama, Masaki; Suzui, Mitsukazu; Asano, Toshifumi; Ishizuka, Toru; Yawo, Hiromu.

BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. 2012. p. 143-148 (BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Urisu, T, Uno, H, Wang, ZH, Obuliraj, SK, Takada, N, Aoyama, M, Suzui, M, Asano, T, Ishizuka, T & Yawo, H 2012, Incubation type planar patch clamp biosensor: Basic performances. in BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices, pp. 143-148, International Conference on Biomedical Electronics and Devices, BIODEVICES 2012, Vilamoura, Algarve, Portugal, 12/2/1.

Urisu, Tsuneo ; Uno, Hidetaka ; Wang, Zhi Hong ; Obuliraj, Senthil Kumar ; Takada, Noriko ; Aoyama, Masaki ; Suzui, Mitsukazu ; Asano, Toshifumi ; Ishizuka, Toru ; Yawo, Hiromu. / Incubation type planar patch clamp biosensor : Basic performances. BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices. 2012. pp. 143-148 (BIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices).

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abstract = "The biosensors based on the incubation type planar patch clamp method was developed and the basic properties were investigated. Usefulness of light-gated ionchannel method on the performance of the device was confirmed. The excess current noise and the thermal noise due to the micropore resistance and the seal resistance were the main sources of the noise, and the noise level of the developed biosensor was 7 pA at the 1 kHz low pass filter. This value is slightly larger than the single ionchannel current level (∼4pA) of TRPV1. We consider that the developed device has a sufficient performance for the whole cell measurements, and extremely suitable for the high throughput screening application with neural network, in which incubation function is essentially necessary.",

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author = "Tsuneo Urisu and Hidetaka Uno and Wang, {Zhi Hong} and Obuliraj, {Senthil Kumar} and Noriko Takada and Masaki Aoyama and Mitsukazu Suzui and Toshifumi Asano and Toru Ishizuka and Hiromu Yawo",

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Incubation type planar patch clamp biosensor: Basic performances

Abstract

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Keywords

ASJC Scopus subject areas

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