Incubation type planar patch clamp biosensor: Basic performances

Tsuneo Urisu, Hidetaka Uno, Zhi Hong Wang, Senthil Kumar Obuliraj, Noriko Takada, Masaki Aoyama, Mitsukazu Suzui, Toshifumi Asano, Toru Ishizuka, Hiromu Yawo

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    2 Citations (Scopus)

    Abstract

    The biosensors based on the incubation type planar patch clamp method was developed and the basic properties were investigated. Usefulness of light-gated ionchannel method on the performance of the device was confirmed. The excess current noise and the thermal noise due to the micropore resistance and the seal resistance were the main sources of the noise, and the noise level of the developed biosensor was 7 pA at the 1 kHz low pass filter. This value is slightly larger than the single ionchannel current level (∼4pA) of TRPV1. We consider that the developed device has a sufficient performance for the whole cell measurements, and extremely suitable for the high throughput screening application with neural network, in which incubation function is essentially necessary.

    Original languageEnglish
    Title of host publicationBIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices
    Pages143-148
    Number of pages6
    Publication statusPublished - 2012 Jun 13
    EventInternational Conference on Biomedical Electronics and Devices, BIODEVICES 2012 - Vilamoura, Algarve, Portugal
    Duration: 2012 Feb 12012 Feb 4

    Publication series

    NameBIODEVICES 2012 - Proceedings of the International Conference on Biomedical Electronics and Devices

    Other

    OtherInternational Conference on Biomedical Electronics and Devices, BIODEVICES 2012
    CountryPortugal
    CityVilamoura, Algarve
    Period12/2/112/2/4

    Keywords

    • Biosensor
    • HEK293
    • Ionchannel
    • PMMA
    • Planar patch clamp
    • TRPV1

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Electrical and Electronic Engineering

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