Increased susceptibility to ischemia-induced brain damage in transgenic mice overexpressing a dominant negative form of SHP2

Yoko Aoki, Zhihong Huang, Sunu S. Thomas, Pradeep G. Bhide, Ivana Huang, Michael A. Moskowitz, Steven A. Reeves

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Cell culture studies have established SH2 domain-containing protein tyrosine phosphatase-2 (SHP2) as an important factor in growth factor and cytokine-activated signaling pathways. However, the significance of SHP2 in the mammalian central nervous system (CNS) is not known since early embryonic lethality occurs in shp2 null mice. To bypass this embryonic lethality, transgenic animals containing a catalytically inactive mutant of SHP2 (SHP2-CS) under the control of a nestin intron II/thymidine kinase minimal promoter were generated. In the developing CNS of these animals, although high-level transgene expression was detected in the neuroepithelium, there was no obvious abnormality in progenitor cell proliferation or migration. In the adult brain, high-level transgene expression was detected in the subventricular zone, rostral migratory stream, dentate gyms of hippocampus, and cerebellum. Because SHP2 function is likely important in cell survival pathways, we used a focal cerebral ischemia model to examined whether SHP2 is important during CNS injury. Ischemia-induced damage and neuronal death was found to be significantly greater in nestin-SHP2-CS mice than in wild-type littermates. These findings indicate that SHP2 is a required factor in signaling pathway(s) important for neuronal survival.

Original languageEnglish
Pages (from-to)1965-1973
Number of pages9
JournalFASEB Journal
Volume14
Issue number13
DOIs
Publication statusPublished - 2000

Keywords

  • Cell survival
  • Cerebral ischemia
  • Neuron
  • Protein tyrosine phosphatase

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Increased susceptibility to ischemia-induced brain damage in transgenic mice overexpressing a dominant negative form of SHP2'. Together they form a unique fingerprint.

Cite this