Increased susceptibility of MER5 (peroxiredoxin III) knockout mice to LPS-induced oxidative stress

Lianqin Li, Wataru Shoji, Hirohisa Takano, Noriko Nishimura, Yasunobu Aoki, Ryoya Takahashi, Sataro Goto, Tomonori Kaifu, Toshiyuki Takai, Masuo Obinata

Research output: Contribution to journalArticlepeer-review

121 Citations (Scopus)

Abstract

MER5 (also called peroxiredoxin III, PrxIII) is a member of peroxiredoxin family that has antioxidant activity. The present study was performed to investigate its in vivo function using MER5 knockout mice. MER5 knockout mice were born in normal frequency and could grow to maturity, but we found that intracellular ROS levels are significantly higher in the macrophages of the knockout mice. We examined roles of MER5 function for the oxidative stress responses by intratracheal inoculation of lipopolysaccharide (LPS) to the mice. Lung inflammation such as inflammatory cell infiltration and airway wall thickening was more severely detected in the knockout mice. At the same time, oxidative damage on DNA and proteins was more strongly detected in lung tissues of the knockout mice, including 8-hydroxy-2′-deoxyguanosine (8-OHdG) formation and protein carbonylation. The degrees of lung inflammation and oxidative damage were positively related with LPS doses. Our results indicate that MER5 knockout mice accumulated higher intracellular ROS levels, which cause LPS-induced lung injury more severely, and thus, suggested that MER5 acts as an important scavenger of reactive oxygen species (ROS) under oxidative stress.

Original languageEnglish
Pages (from-to)715-721
Number of pages7
JournalBiochemical and biophysical research communications
Volume355
Issue number3
DOIs
Publication statusPublished - 2007 Apr 13

Keywords

  • Lung inflammation
  • MER5 (peroxiredoxin III)
  • Oxidative stress
  • Peroxiredoxin
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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