Increased production of tumor necrosis factor-α by peritoneal fluid mononuclear cells induced by 60-kDa heat shock protein in women with minimal to mild endometriosis

Takahiro Noda, Takashi Murakami, Yukihiro Terada, Nobuo Yaegashi, Kunihiro Okamura

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Problem: We sought to examine the role of Hsp60, a 60-kDa stress-responsive protein (hsp60) with potent immunostimulatory properties, in the pathophysiology of endometriosis. We investigated the response of peritoneal fluid mononuclear cells (PFMCs) to hsp60 to identify differences in PFMCs between women with and without endometriosis. Method of study: PFMCs from women with or without endometriosis were incubated with hsp60 in vitro. The release of tumor necrosis factor (TNF)-ot was measured by enzyme-linked immunosorbent assay (ELISA). Results: The rapid secretion of TNF-α was observed following the exposure of PFMCs to hsp60. PFMCs from women with minimal to mild endometriosis produced greater amounts of TNF-α in response to hsp60 stimulation than levels seen in cells from women without endometriosis. Conclusion: Hsp60 may play a role in the activation of peritoneal immunity in patients with minimal to mild endometriosis.

Original languageEnglish
Pages (from-to)427-432
Number of pages6
JournalAmerican Journal of Reproductive Immunology
Volume50
Issue number5
DOIs
Publication statusPublished - 2003 Nov

Keywords

  • Endometriosis
  • Heat shock protein 60
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

Fingerprint Dive into the research topics of 'Increased production of tumor necrosis factor-α by peritoneal fluid mononuclear cells induced by 60-kDa heat shock protein in women with minimal to mild endometriosis'. Together they form a unique fingerprint.

Cite this