Increased production of reactive oxygen species by the vacuolar-type (H+)-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells

Aya Yokomakura, Jang Ja Hong, Kazuo Ohuchi, Seong Eun Oh, Jin Yong Lee, Nariyasu Mano, Tsutomu Takahashi, Gi Wook Hwang, Akira Naganuma

Research output: Contribution to journalLetterpeer-review

5 Citations (Scopus)

Abstract

Treatment of the mouse leukemic cell line RAW 264 with bafilomycin A1 or concanamycin A, inhibitors of vacuolar-type (H+)-ATPases (V-ATPases), significantly increased the production of reactive oxygen species (ROS) and decreased cell viability. These effects were significantly suppressed by the presence of N-acetyl cysteine (NAC), an ROS scavenger. si-RNA mediated knockdown of the gene for the c subunit of the V0 domain of V-ATPase also resulted in an increase in ROS production and a decrease in cell viability. These results suggest that decreased cellular V-ATPase activity decreases cell viability by increasing ROS production in RAW 264 cells.

Original languageEnglish
Pages (from-to)1045-1048
Number of pages4
JournalJournal of Toxicological Sciences
Volume37
Issue number5
DOIs
Publication statusPublished - 2012 Oct

Keywords

  • Bafilomycin A1
  • Concanamycin A
  • RAW 264 cells
  • Reactive oxygen species
  • V-ATPase inhibitor

ASJC Scopus subject areas

  • Toxicology

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