Increased production of biomass-degrading enzymes by double deletion of creA and creB genes involved in carbon catabolite repression in Aspergillus oryzae

Sakurako Ichinose, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    In a previous study, we reported that a double gene deletion mutant for CreA and CreB, which constitute the regulatory machinery involved in carbon catabolite repression, exhibited improved production of α-amylase compared with the wild-type strain and single creA or creB deletion mutants in Aspergillus oryzae. Because A. oryzae can also produce biomass-degrading enzymes, such as xylolytic and cellulolytic enzymes, we examined the production levels of those enzymes in deletion mutants in this study. Xylanase and β-glucosidase activities in the wild-type were hardly detected in submerged culture containing xylose as the carbon source, whereas those enzyme activities were significantly increased in the single creA deletion (ΔcreA) and double creA and creB deletion (ΔcreAΔcreB) mutants. In particular, the ΔcreAΔcreB mutant exhibited >100-fold higher xylanase and β-glucosidase activities than the wild-type. Moreover, in solid-state culture, the β-glucosidase activity of the double deletion mutant was >7-fold higher than in the wild-type. These results suggested that deletion of both creA and creB genes could also efficiently improve the production levels of biomass-degrading enzymes in A. oryzae.

    Original languageEnglish
    Pages (from-to)141-147
    Number of pages7
    JournalJournal of Bioscience and Bioengineering
    Volume125
    Issue number2
    DOIs
    Publication statusPublished - 2018 Feb

    Keywords

    • Aspergillus oryzae
    • Carbon catabolite derepression
    • Gene deletion
    • Increased enzyme production
    • Xylanase
    • β-Glucosidase

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

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