Increased positive selection pressure within the complementarity determining regions of the T-cell receptor β gene in New World monkeys

Takaji Matsutani, Yoshiki Fujii, Kazutaka Kitaura, Satsuki Suzuki, Yuji Tsuruta, Tomohiko Takasaki, Kouetsu Ogasawara, Norihiro Nishimoto, Ichiro Kurane, Ryuji Suzuki

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Because of the long-term co-evolution of TCR and MHC molecules, numerous nucleotide substitutions have accumulated within the domains of TCRβ genes. We previously found that nonsynonymous nucleotide substitutions occurred more frequently in complementarity determining region (CDR)β than in CDRα, even though only a limited number of common marmoset (Callithrix jacchus) and human T-cell receptor β variable (TRBV) sequences were compared. This interesting finding raised the question of whether the increased selective pressure within CDRβ was species-specific. In this study, we identified 21 TRBV region sequences from the common marmoset and performed comparative sequence analyses of the T-cell receptor α variable (TRAV) and TRBV regions from human, chimpanzee, rhesus monkey, cotton-top tamarin, Ma's night monkey, and common marmoset. The ratios of the number of nonsynonymous nucleotide substitutions per site (d N) to the d S values (d N/d S) were less than 1 within the framework regions (FRs) of TRAV and TRBV region sequences, suggesting that purifying selection is largely dominant within the FRs. In contrast, the d N values were statistically significantly greater for CDRβ than for CDRα only in New World monkeys. Also, increased d N/d S ratios (d N/d S>1) were observed within CDRβ between humans and New World monkeys and, interestingly, between New World monkeys, which share a relatively recent common ancestor. Moreover, phylogenetic analysis by maximum likelihood analysis provided firm evidence to support that positive selection occurred within CDRβ along New World monkey lineages. These results suggest that increased positive selection pressure within CDRβ is common in New World monkeys rather than being species-specific. This study provides an intriguing insight into the co-evolution of TCR and MHC molecules within primates.

Original languageEnglish
Pages (from-to)1082-1092
Number of pages11
JournalAmerican Journal of Primatology
Volume73
Issue number10
DOIs
Publication statusPublished - 2011 Oct

Keywords

  • Common marmoset
  • New World monkey
  • Positive selection
  • Primate evolution
  • T-cell receptor

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

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