Increased mRNA for low density lipoprotein receptor in livers of rabbits treated with 17α-ethinyl estradiol

P. T.S. Ma, T. Yamamoto, J. L. Goldstein, M. S. Brown

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Abstract

Pharmacologic doses of 17α-ethinyl estradiol are known to increase the number of low density lipoprotein (LDL) receptors in livers of rats, thereby producing a profound fall in plasma cholesterol levels. We now report that ethinyl estradiol exerts the same effect in livers of male and female rabbits and that the increase in receptor number is correlated with a 6- to 8-fold increase in the levels of receptor mRNA. Receptor protein was measured by ligand blotting, and mRNA levels were measured by a quantitative solution hybridization/S1 nuclease protection assay using uniformly 32P-labeled single-stranded cDNA probes. These experiments demonstrate that pharmacologic induction of the mRNA for the LDL receptor in liver can lead to increased LDL receptor levels and a fall in plasma cholesterol in experimental animals.

Original languageEnglish
Pages (from-to)792-796
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number3
DOIs
Publication statusPublished - 1986 Jan 1

ASJC Scopus subject areas

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