Increased monocytic adhesion by senescence in human umbilical vein endothelial cells

Miyuki Yanaka, Taro Honma, Kenta Sato, Nahoko Shinohara, Junya Ito, Yurie Tanaka, Tsuyoshi Tsuduki, Ikuo Ikeda

    Research output: Contribution to journalArticlepeer-review

    26 Citations (Scopus)

    Abstract

    We investigated whether replicative senescence of endothelial cells contributed to the pathogenesis of atherosclerosis in human umbilical vein endothelial cells (HUVECs). HUVECs at a population-doubling level of 30 (PDL30) divided much more slowly than those at PDL9. The percentage of SA-β-Gal-positive cells and the mRNA expression levels of PAI-1 and p21 at PDL30 were significantly higher than those at PDL9. The changes induced by aging were evaluated according to the mRNA expression level of genes related to the endothelial cell function. The expression level of many adhesion molecules promoting monocytic adhesion was significantly increased, and monocytic adhesion on HUVECs was found to be significantly promoted by aging. Monocytic adhesion is an essential early event in the development of atherosclerosis, and our results suggest that replicative senescence of the vascular endothelial cells induced increased expression of adhesion molecules. The consequent increase in monocytic adhesion may then promote the pathogenesis of atherosclerosis.

    Original languageEnglish
    Pages (from-to)1098-1103
    Number of pages6
    JournalBioscience, Biotechnology and Biochemistry
    Volume75
    Issue number6
    DOIs
    Publication statusPublished - 2011

    Keywords

    • Aging
    • Cell adhesion
    • Human umbilical vein endothelial cell
    • Monocytic adhesion
    • Senescence

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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