Increased monocytic adhesion by senescence in human umbilical vein endothelial cells

Miyuki Yanaka, Taro Honma, Kenta Sato, Nahoko Shinohara, Junya Ito, Yurie Tanaka, Tsuyoshi Tsuduki, Ikuo Ikeda

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

We investigated whether replicative senescence of endothelial cells contributed to the pathogenesis of atherosclerosis in human umbilical vein endothelial cells (HUVECs). HUVECs at a population-doubling level of 30 (PDL30) divided much more slowly than those at PDL9. The percentage of SA-β-Gal-positive cells and the mRNA expression levels of PAI-1 and p21 at PDL30 were significantly higher than those at PDL9. The changes induced by aging were evaluated according to the mRNA expression level of genes related to the endothelial cell function. The expression level of many adhesion molecules promoting monocytic adhesion was significantly increased, and monocytic adhesion on HUVECs was found to be significantly promoted by aging. Monocytic adhesion is an essential early event in the development of atherosclerosis, and our results suggest that replicative senescence of the vascular endothelial cells induced increased expression of adhesion molecules. The consequent increase in monocytic adhesion may then promote the pathogenesis of atherosclerosis.

Original languageEnglish
Pages (from-to)1098-1103
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number6
DOIs
Publication statusPublished - 2011

Keywords

  • Aging
  • Cell adhesion
  • Human umbilical vein endothelial cell
  • Monocytic adhesion
  • Senescence

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Increased monocytic adhesion by senescence in human umbilical vein endothelial cells'. Together they form a unique fingerprint.

Cite this