TY - JOUR
T1 - Increased hydrostatic pressure induces nuclear translocation of DAF-16/FOXO in C. elegans
AU - Watanabe, Naoshi
AU - Morimatsu, Masatoshi
AU - Fujita, Ayano
AU - Teranishi, Mika
AU - Sudevan, Surabhi
AU - Watanabe, Masaru
AU - Iwasa, Hiroaki
AU - Hata, Yutaka
AU - Kagi, Hiroyuki
AU - Nishiyama, Masayoshi
AU - Naruse, Keiji
AU - Higashitani, Atsushi
PY - 2020/3/19
Y1 - 2020/3/19
N2 - Mechanical stimulation is well known to be important for maintaining tissue and organ homeostasis. Here, we found that hydrostatic pressure induced nuclear translocation of a forkhead box O (FOXO) transcription factor DAF-16, in C. elegans within minutes, whereas the removal of this pressure resulted in immediate export of DAF-16 to the cytoplasm. We also monitored DAF-16-dependent transcriptional changes by exposure to 1 MPa pressure for 5 min, and found significant changes in collagen and other genes in a DAF-16 dependent manner. Lifespan was markedly prolonged with exposure to cyclic pressure treatment (1 MPa once a day for 5 min from L1 larvae until death). Furthermore, age-dependent decline in locomotor activity was suppressed by the treatment. In contrast, the nuclear translocation of the yes-associated protein YAP-1 was not induced under the same pressure conditions. Thus, moderate hydrostatic pressure improves ageing progression through activation of DAF-16/FOXO in C. elegans.
AB - Mechanical stimulation is well known to be important for maintaining tissue and organ homeostasis. Here, we found that hydrostatic pressure induced nuclear translocation of a forkhead box O (FOXO) transcription factor DAF-16, in C. elegans within minutes, whereas the removal of this pressure resulted in immediate export of DAF-16 to the cytoplasm. We also monitored DAF-16-dependent transcriptional changes by exposure to 1 MPa pressure for 5 min, and found significant changes in collagen and other genes in a DAF-16 dependent manner. Lifespan was markedly prolonged with exposure to cyclic pressure treatment (1 MPa once a day for 5 min from L1 larvae until death). Furthermore, age-dependent decline in locomotor activity was suppressed by the treatment. In contrast, the nuclear translocation of the yes-associated protein YAP-1 was not induced under the same pressure conditions. Thus, moderate hydrostatic pressure improves ageing progression through activation of DAF-16/FOXO in C. elegans.
KW - Ageing
KW - Longevity
KW - Mechanical stimulation
KW - Mega pascal
KW - Transcription factor
UR - http://www.scopus.com/inward/record.url?scp=85077919797&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85077919797&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2020.01.047
DO - 10.1016/j.bbrc.2020.01.047
M3 - Article
C2 - 31954516
AN - SCOPUS:85077919797
VL - 523
SP - 853
EP - 858
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -