TY - JOUR
T1 - Increased HMGB1 levels in lesional skin and sera in patients with cutaneous T-cell lymphoma
AU - Senda, Naoyuki
AU - Miyagaki, Tomomitsu
AU - Kamijo, Hiroaki
AU - Nakajima, Rina
AU - Oka, Tomonori
AU - Takahashi, Naomi
AU - Suga, Hiraku
AU - Yoshizaki, Ayumi
AU - Asano, Yoshihide
AU - Sugaya, Makoto
AU - Sato, Shinichi
N1 - Funding Information:
Disclosure. Financial support: this study was supported by the Japan Society for the Promotion of Science KAKENHI Grant. Conflicts of interest: none. Acknowledgements: we thank Tamami Kaga for technical assistance.
Publisher Copyright:
© 2018, John Libbey Eurotext.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Background: High mobility group box-1 (HMGB1) is a ubiquitously expressed non-histone nuclear protein which acts as a danger signal when released from cells. HMGB1, which is associated with inflammation, angiogenesis, and T helper (Th)2 polarization, contributes to the development of various inflammatory diseases and malignancies. However, it remains to be determined whether HMGB1 is involved in cutaneous Tcell lymphoma (CTCL). Objectives: To investigate the role of HMGB1 in CTCL. Materials & methods: Serum HMGB1 levels were measured in patients with CTCL and healthy controls using an enzyme-linked immunosorbent assay. HMGB1 messenger RNA (mRNA) expression in CTCL and normal skin was examined by quantitative reverse transcription polymerase chain reaction and immunohistochemistry. Moreover, we analysed correlations between the levels of HMGB1 and other cytokines and chemokines, laboratory data, disease activity, and prognosis. Results: HMGB1 levels were significantly higher in sera of CTCL patients than those of normal controls and correlated with serum levels of soluble interleukin-2 receptor, lactate dehydrogenase, thymus and activation-regulated chemokine, and the number of eosinophils in peripheral blood. Serum HMGB1 levels also reflected disease activity and prognosis for each patient with CTCL. HMGB1 mRNA levels in CTCL lesional skin were significantly elevated and correlated with IL- 4, IL-10, IL-19, and angiogenin mRNA levels. Immunohistochemical staining revealed that HMGB1 was expressed not only in the nucleus but also in the cytoplasm of various cells in CTCL lesional skin. Conclusion: These results suggest that enhanced HMGB1 expression may contribute to the progression of CTCL through Th2 polarization and promotion of angiogenesis.
AB - Background: High mobility group box-1 (HMGB1) is a ubiquitously expressed non-histone nuclear protein which acts as a danger signal when released from cells. HMGB1, which is associated with inflammation, angiogenesis, and T helper (Th)2 polarization, contributes to the development of various inflammatory diseases and malignancies. However, it remains to be determined whether HMGB1 is involved in cutaneous Tcell lymphoma (CTCL). Objectives: To investigate the role of HMGB1 in CTCL. Materials & methods: Serum HMGB1 levels were measured in patients with CTCL and healthy controls using an enzyme-linked immunosorbent assay. HMGB1 messenger RNA (mRNA) expression in CTCL and normal skin was examined by quantitative reverse transcription polymerase chain reaction and immunohistochemistry. Moreover, we analysed correlations between the levels of HMGB1 and other cytokines and chemokines, laboratory data, disease activity, and prognosis. Results: HMGB1 levels were significantly higher in sera of CTCL patients than those of normal controls and correlated with serum levels of soluble interleukin-2 receptor, lactate dehydrogenase, thymus and activation-regulated chemokine, and the number of eosinophils in peripheral blood. Serum HMGB1 levels also reflected disease activity and prognosis for each patient with CTCL. HMGB1 mRNA levels in CTCL lesional skin were significantly elevated and correlated with IL- 4, IL-10, IL-19, and angiogenin mRNA levels. Immunohistochemical staining revealed that HMGB1 was expressed not only in the nucleus but also in the cytoplasm of various cells in CTCL lesional skin. Conclusion: These results suggest that enhanced HMGB1 expression may contribute to the progression of CTCL through Th2 polarization and promotion of angiogenesis.
KW - HMGB1
KW - Th2 polarization
KW - angiogenesis
KW - cutaneous T-cell lymphoma
KW - mycosis fungoides
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U2 - 10.1684/ejd.2018.3400
DO - 10.1684/ejd.2018.3400
M3 - Article
C2 - 30378541
AN - SCOPUS:85058594897
SN - 1167-1122
VL - 28
SP - 621
EP - 627
JO - European Journal of Dermatology
JF - European Journal of Dermatology
IS - 5
ER -