Increased expression of PDGF A- and B-chain genes in rat lungs with hypoxic pulmonary hypertension

D. Katayose, M. Ohe, K. Yamauchi, M. Ogata, K. Shirato, H. Fujita, S. Shibahara, T. Takishima

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101 Citations (Scopus)

Abstract

To study the molecular basis of vascular remodeling in pulmonary hypertension, we developed an experimental system in which male Sprague- Dawley rats were exposed to hypoxia for up to 3 wk. Both the right ventricular systolic pressure and gravimetric index for right ventricular hypertrophy were higher in rats exposed to hypoxia for 3 wk than those of age-matched control rats (P < 0.01), indicating that pulmonary hypertension was established under conditions used. To examine the possible involvement of platelet-derived growth factor (PDGF) in the pulmonary vascular remodeling caused by hypoxia, we cloned rat PDGF A- and B-chain cDNA and prepared specific cRNA probes. Northern blot analysis revealed that PDGF B-chain mRNA levels in the lungs were increased, reached a maximum of day 1, and were sustained at day 3, whereas PDGF A-chain mRNA levels reached a maximum on day 3. Thus the increase in the PDGF B-chain mRNA level precedes that in the PDGF A-chain mRNA level. These results suggest that the PDGF A- and B-chain products may be coordinately and sequentially involved in hypoxic pulmonary vascular remodeling.

Original languageEnglish
Pages (from-to)L100-L106
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume264
Issue number2 8-2
DOIs
Publication statusPublished - 1993
Externally publishedYes

Keywords

  • brain
  • hypoxia
  • molecular cloning
  • platelet-derived growth factor
  • vascular remodeling

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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