Increased expression of nucleoside diphosphate kinases/nm23 in human diploid fibroblasts transformed by SV40 large T antigen or 60Co irradiation

Kinuko Ohneda, Mitsugu Fukuda, Nobuko Shimada, Naoshi Ishikawa, Tateo Ichou, Kazuhiko Kaji, Takayoshi Toyota, Narimichi Kimura

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

When the expression levels of nucleoside diphosphate (NDP) kinase/nm23 were examined in four human normal diploid fibroblast cell lines in comparison with their corresponding immortalized cells transformed by SV40 large T antigen or 60Co irradiation, mRNA levels of the two isoforms (NDP kinase A/nm23-H1, NDP kinase B/nm23-H2) were increased in the immortalized cell lines. The increase was found to be associated with increased translation products. Furthermore, the cell extracts prepared from these immortalized cell lines demonstrated slightly higher enzyme activity than those from their normal counterparts. Neither the growth state nor the in vitro aging largely affected their expression in a normal cell line (TIG-3) examined. The results suggest possible involvement of NDP kinases/nm23 in acquiring an infinite growth property of these cells.

Original languageEnglish
Pages (from-to)273-277
Number of pages5
JournalFEBS Letters
Volume348
Issue number3
DOIs
Publication statusPublished - 1994 Jul 18

Keywords

  • Enzyme activity
  • Gene expression
  • Human diploid cells
  • Immortalization
  • NDP kinase
  • nm23

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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