Increased expression of heme oxygenase mRNA in rat brain following transient forebrain ischemia

Atsushi Takeda, Hiroshi Onodera, Akira Sugimoto, Yasuto Itoyama, Kyuya Kogure, Shigeki Shibahara

Research output: Contribution to journalArticlepeer-review

86 Citations (Scopus)


Heme oxygenase is a rate-limiting enzyme in heme catabolism, the end products of which include iron, carbon monoxide and bilirubin. Expression of the inducible form of heme oxygenase (HO-1) was investigated in rat brain following 20 min of forebrain ischemia by Northern blot and in situ hybridization analyses. The level of HO-1 mRNA was undetectable in the cerebral cortex of sham control, but increased following ischemic insult, reached the maximum after 12 h of reperfusion, and then decreased. In sham control brain, HO-1 mRNA was detectable only in the scattered neuron-like cells within the dentate gyrus hilus. At 12 h of reperfusion, the remarkable increase in HO-1 mRNA levels was observed in both neuronal and glia-like cells disturbed in the neocortex, hippocampus and thalamus.

Original languageEnglish
Pages (from-to)120-124
Number of pages5
JournalBrain research
Issue number1
Publication statusPublished - 1994 Dec 12
Externally publishedYes


  • Bilirubin
  • Cytoprotection
  • Gene expression
  • Heat shock protein

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


Dive into the research topics of 'Increased expression of heme oxygenase mRNA in rat brain following transient forebrain ischemia'. Together they form a unique fingerprint.

Cite this