Increased enzyme production under liquid culture conditions in the industrial fungus Aspergillus oryzae by disruption of the genes encoding cell wall α-1,3-glucan synthase

Ken Miyazawa, Akira Yoshimi, Silai Zhang, Motoaki Sano, Mayumi Nakayama, Katsuya Gomi, Keietsu Abe

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Under liquid culture conditions, the hyphae of filamentous fungi aggregate to form pellets, which reduces cell density and fermentation productivity. Previously, we found that loss of α-1,3-glucan in the cell wall of the fungus Aspergillus nidulans increased hyphal dispersion. Therefore, here we constructed a mutant of the industrial fungus A. oryzae in which the three genes encoding α-1,3-glucan synthase were disrupted (tripleΔ). Although the hyphae of the tripleΔ mutant were not fully dispersed, the mutant strain did form smaller pellets than the wild-type strain. We next examined enzyme productivity under liquid culture conditions by transforming the cutinase-encoding gene cutL1 into A. oryzae wildtype and the tripleΔ mutant (i.e. wild-type-cutL1, tripleΔ-cutL1). A. oryzae tripleΔ-cutL1 formed smaller hyphal pellets and showed both greater biomass and increased CutL1 productivity compared with wild-type-cutL1, which might be attributable to a decrease in the number of tripleΔ-cutL1 cells under anaerobic conditions.

Original languageEnglish
Pages (from-to)1853-1863
Number of pages11
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number9
DOIs
Publication statusPublished - 2016 Jan 1

Keywords

  • Aspergillus
  • Cell wall
  • Enzyme
  • Production
  • α-1,3-glucan

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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