Increase of c-Fos and c-Jun expression in spinal and cranial motoneurons of the degenerating muscle mouse (Scn8admu)

Hiroyuki Ichikawa, Mitsuhiro Kano, Yoshinaka Shimizu, Toshihiko Suzuki, Eri Sawada, Wako Ono, Leona W.G. Chu, Patrice D. Côté

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The degenerating muscle (dmu) mouse harbors a loss-of-function mutation in the Scn8a gene, which encodes the α subunit of the voltage-gated sodium channel (VGSC) NaV1.6. The distribution of c-Fos and c-Jun was examined in spinal and cranial motoneurons of the dmu mouse. In the cervical spinal cord, trigeminal motor nucleus (Vm), facial nucleus (VII), dorsal motor nucleus of the vagus (X), and hypoglossal nucleus (XII) of wild-type mice, motoneurons expressed c-Fos and c-Jun-immunoreactivity. The immunoreactivity in wild-type mice was mostly weak and localized to the nucleus of these neurons whereas in the spinal cord and brain stem of dmu mice motoneurons showed intense c-Fos and c-Jun-immunoreactivity. The number of c-Fos-immunoreactive motoneurons was dramatically elevated in the cervical spinal cord (wild type, 4.8 ± 1.0; dmu, 17.3 ± 1.6), Vm (wild type, 76.2 ± 21.6; dmu, 216.9 ± 30.9), VII (wild type, 162.4 ± 43.3; dmu, 533.3 ± 41.2), and XII (wild type, 58.2 ± 43.3; dmu, 150.9 ± 25.7). The mutation also increased the number of c-Jun-immunoreactive motoneurons in the cervical spinal cord (wild type, 1.6 ± 0.8; dmu, 12.1 ± 2.1), Vm (wild type, 41.4 ± 18.0; dmu, 123.1 ± 11.7), and X (wild type, 39.1 ± 10.7; dmu, 92.8 ± 17.8). The increase of these transcription factors may be associated with the uncoordinated and excessive movement of forelimbs and degeneration of cardiac muscles in dmu mice.

Original languageEnglish
Pages (from-to)737-742
Number of pages6
JournalCellular and molecular neurobiology
Volume30
Issue number5
DOIs
Publication statusPublished - 2010 Jul

Keywords

  • Brain stem
  • Dmu mouse
  • Immunohistochemistry
  • Motoneuron
  • Nav 1.6
  • Scn8a
  • Spinal cord
  • Voltage-gated sodium channel
  • c-Fos
  • c-Jun

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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