Increase in cytoplasmic free calcium concentration initiated by T3 antigen stimulation is imparied in adult T-cell leukemia cells

Shigeki Tamori, Takashi Uchiyama, Hiroshi Umadome, Toshiyuki Hori, Haruto Uchino, Toshio Hattori, Koichi Araki

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We studied the change in cytoplasmic free calcium ion concentration ([Ca2+]i) in the peripheral blood leukemic cells from adult T-cell leukemia (ATL) patients stimulated by anti-T3(CD3) or anti-T11(CD2) antibodies in order to see whether there is an abnormal response in the early activation processes following T3 or T11 antigen stimulation. Peripheral blood mononuclear cells (PBMC) from four T-cell chronic lymphocytic leukemia (T-CLL) patients showed a rapid and clear increase in [Ca2+]i (from 165 ± 26 nM to more than 299 nM) when stimulated by OKT3 antibody and anti-mouse IgG antibody. This response was comparable to that of PBMC from 10 normal individuals (from 151 ± 20 nM to 252 ± 34 nM). In contrast, PBMC from 10 ATL patients showed only a slight increase in [Ca2+]i (from 137 ± 21 nM to 176 ± 32 nM) following T3 stimulation. The experiments with higher concentrations of OKT3 antibody suggested that this attenuated increase in [Ca2+]i in ATL cells was not exclusively due to impaired expression of T3 antigen. The [Ca2+]i increase in ATL cells induced by the stimulation with two anti-T11 antibodies recognizing different epitopes of the T11 antigen, however, was comparable to that of normal PBMC. The abnormal response of [Ca2+]i to the T-cell receptor /T3 antigen stimulation in ATL may be related to dysfunction or leukemogenesis of HTLV-I-infected cells.

Original languageEnglish
Pages (from-to)357-363
Number of pages7
JournalLeukemia Research
Volume12
Issue number4
DOIs
Publication statusPublished - 1988

Keywords

  • ATL
  • CD2(T11) antigen
  • CD3(T3) antigen
  • Quin2
  • T-CLL
  • cytoplasmic free calcium concentration

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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